T-Cell Activation

Imaging protocol for super-resolution microscopy in fixed T cells.- Visualization and quantification of NFAT1 translocation in T cells.- Deep Learning-Based Image Restoration and Super-Resolution for Fluorescence Microscopy: Overview and Resources.- Analysing Point Accumulation in Nanoscale Topography (PAINT) data to decipher T cell receptor cluster signalling.- Measuring NAADP-evoked Ca2+ release in permeabilized T cells.- An improved two-dimensional HPLC method for endogenous 2′-deoxy-ADPR and 2′-deoxy-NAD.- Analysis of Store-Operated Ca2+ Entry in primary T cells.- Monitoring Dynamic of cAMP Signaling in Primary Murine CD4+ T Cells via Live-Cell Imaging.- Using specific reporter mice to assess CD4+ T cell transdifferentiation.- Generation of antigen-specific CD4+ primary rat T cell lines.- Induction and assessment of activation in rat effector CD4+ memory Th cells.- Imaging BiTE-mediated activation of primary human CD8+ T cells.- Flow cytometric analysis of mouse brain CD8+ T cell activation.- Utilizing pMHC Multimers to Study Antigen-Specific T Cells and T Cell Exhaustion in Chronic Viral Infections.- Analysis of TF/DNA binding in T cells applying Alpha technology.- Using Seahorse technology as an efficient way of verifying T-cell stimulation.- Metabolic screening of T lymphocytes during activation via SEAHORSE extracellular flux (XF) analysis.-  Metabolic profiling of activated T lymphocytes using single-cell energetic metabolism by profiling translation inhibition (SCENITH).