Technical Manual

Collection, processing, storage and shipment of blood and tissues - blood collection, haemapheresis, blood components - preparation, storage and shipment, transfusion-transmitted viruses, cryopreservation of blood, tissue banking and organ transplantation; immunologic and genetic principles - immunohaematology, the antiglobulin test, genetics; blood groups - ABO, H and P blood groups and structurally related antigen, Rh and LW blood groups, other blood groups, the HLA system; application of serologic principles of transfusion practice - pretransfusion testing, identification of unexpected alloantibodies, investigation of a positive DAT and immune haemolysis; clinical considerations in transfusion practices - blood transfusion practice, neonatal and obstetrical transfusion practice, adverse effects of blood transfusion, autologous transfusion; other considerations - safety, quality management, records, blood usage review, blood inventory management; methods - general laboratory, blood grouping and typing, antibody detection and compatibility testing, alloantibody identification and titration, elution, immune haemolysis, autoantibodies and drugs, haemolytic disease of the newborn, HLA typing, infectious disease testing, blood collection storage, and component preparation, quality control.