Intraoperative Flow Cytometry

Georgios Alexiou is an Associate Prof. of Neurosurgery  at the University Hospital of Ioannina, Greece. He undertook his neurosurgical training at ''Agia Sofia'' Children's Hospital in Athens and at the University Hospital of Ioannina, Greece. After finishing his residency, he received the EANS Spine Fellowship award and worked as a Clinical Fellow in the Department of Neurosurgery in Leeds Teaching Hospital, UK. He then received the AANS International Travelling Fellowship in Pediatric Neurosurgery award at the Department of Neurosurgery in Boston Children's Hospital, Harvard Medical School. Dr. Alexiou worked as a consultant at the Gamma Knife Center at the University Hospital La Timone in Marseille, France. He has a Master degree in Biotechnology and Public Health. Dr. Alexiou worked as a postdoctoral research fellow at MD Anderson Cancer Center in Houston Texas and is also working on basic research at the Neurosurgical Institute in Ioannina, Greece.  His primaryresearch interests are pediatric neurosurgery, advanced imaging and radiotherapy techniques to improve the outcomes of glioma patients, intraoperative flow cytometry for brain tumor surgery and on the discovery and validation of novel chemotherapeutic agents for glioma treatment.

Georgios Vartholomatos is a BsC in Biochemistry and PhD in Biochemistry and Molecular Genetics. He has been a visiting post-graduate at the University of California, Berkeley. He is currently the Scientific Correspondent of the Unit of Molecular Biology and Translation Flow Cytometry, University Hospital of Ioannina, Greece, which is reference centre in Epirus region for the diagnosis of haematology malignancies and autoimmune diseases using flow cytometry. He is the Coordinator of the Research Working Group of intraoperative Flow Cytometry (iFC) and has a solid experience in the use of flow cytometry as a diagnostic and research tool, being author of several publications that present innovative flow-cytometric approaches in clinical practice. He is deeply involved in the development and clinical implementation of novel diagnostic intraoperative tools for the cell cycle DNA analysis and immunophenotype in different types of solid tumors. 

I. General Topics.- 1. History of Flow Cytometry.- 2. Basic Principles.- 3. Sample-Data Analysis.- . II. Intraoperative Flow Cytometry.- 4. Basic Principles.- 5. Sample - Data Analysis.- . III. Intraoperative Flow Cytometry in CNS malignancies.- 6. Pathology of the Tumors of the Central Nervous System.- 7. Current methods for intraoperative application.- 8. IFC in Gliomas.- 9. IFC in Meningiomas.- 10. IFC in Pediatric Tumors.- 11. IFC in Spine Tumors.- . IV. Intraoperative Flow Cytometry in Breast malignancies.- 12. Breast Cancer.- 13. Current methods for intraoperative application.- 14. IFC in Lumpectomy.- . V. Intraoperative Flow Cytometry in Head and Neck malignancies.- 15. Head and Neck malignancies.- 16. Current Techniques  for intraoperative application.- 17. IFC in Head and Neck malignancies.- . VI. Intraoperative Flow Cytometry in Gastrointestinal malignancies.- 18. Gastrointestinal Malignancies.- 19. Current methods for intraoperative application.- 20. IFC in liver cancer.- 21. IFC in Colorectal Cancer.- 22. Future Perspectives of IFC.