mRNA Decay

Minimal Perturbation Analysis of mRNA Degradation Rates with Tet Off and RT qPCR.- Northern Blotting: Protocols for Radioactive and Non-Radioactive Detection of RNA.- RNA Blotting by Electrotransfer and RNA Detection.- Dynamic Evolution of Poly-A Tail Lengths Visualized by RNAse H Assay and Northern Blot Using Non-Radioactive Probes in Yeast.- Assessing Deadenylation Activity by Polyacrylamide Gel Electrophoresis Using a Fluorescent RNA Substrate.- Studying Exoribonuclease Activity Using Fluorescence Anisotropy Assay.- Purification of Enzymatically Active Xrn1 for Removal of Non-Capped mRNAs from In Vitro Transcription Reactions and Evaluation of mRNA Decapping Status In Vivo.- Production of Fully Capped mRNA for Transfection into Mammalian Cells: A Protocol for Enzymatic Degradation of Uncapped Transcripts after In Vitro mRNA Synthesis.- Transcriptome-Wide Analysis of the 5' Cap Status of RNA Using 5´Monophosphate-Dependent Exonuclease Digestion and RNA Sequencing.- RNA Decay Assay: 5-Ethynyl-Uridine Labeling and Chasing.- Assessment of mRNA Decay and Calculation of Codon Occurrence to mRNA Stability Correlation Coefficients after 5-EU Metabolic Labeling.- Measurement of rRNA Synthesis and Degradation Rates by 3H-Uracil Labeling in Yeast.- A Method for Rapid Inducible RNA Decay.- reCRAC: A Stringent Method for Precise Mapping of Protein-RNA Interactions in Yeast.- Snapchat Moments of the Cell Revealed by UV-Crosslinking and RNA Co-Immunoprecipitation of Transient RNA-Protein Complexes.- Using the Tether Function Assay to Identify Potential Regulators of mRNA Translation and mRNA Decay.- Nascent and Mature RNA Profiling by Subcellular Fractionation in Human Cells.- Concurrent Profiling of Localized Transcriptome and RNA Dynamics in Neurons by Spatial SLAMseq.- Analysis of Cytoplasmic RNA Decay Targets Using the Auxin Degron System.- Detection of Nuclear RNA Decay Intermediates Using a Modified Oxford Nanopore RNA Sequencing Strategy.- Defining the True Native Ends of RNAs at Single-Molecule Level with TERA-Seq.- Global Profiling and Analysis of 5' Monophosphorylated mRNA Decay Intermediates.- SelectRepair Knockout: Efficient PTC-Free Gene Knockout Through Selectable Homology-Directed DNA Repair.