Handbook of ELISPOT

Important Considerations for Elispot Validation.- Current Trends in Validating Antibody Specificities for Elispot by Western Blotting.- An Overview of Peptides and Peptide Pools for Antigen-Specific Stimulation in T-Cell Assays.- The Applications of ELISpot in the Identification and Treatment of Various Forms of Tuberculosis and in the Cancer Immunotherapies.- Artificial Intelligence-Based Counting Algorithm Enables Accurate and Detailed Analysis of the Broad Spectrum of Spot Morphologies Observed in Antigen-Specific B Cell ELISPOT and FluoroSpot Assays.- Comparing Flow Cytometry and ELISpot for Detection of IL-10, IL-6, and TNF Alpha on Human PBMCs.- Reagent Tracker™ Platform Verifies and Provides Audit Trails for the Error-Free Implementation of T Cell ImmunoSpot® Assays.- Detection of SARS-CoV-2-Specific Cells Utilizing Whole Proteins and/or Peptides in Human PBMCs using IFN-ƴ ELISpot Assay.- Interferon-γ/IL-2 ELISpot and mRNA Responses to the SARS-CoV2, Feline Coronavirus Serotypes 1 (FCoV1), and FCoV2 Receptor Binding Domains by the T Cells from COVID-19-Vaccinated Humans and FCoV1-Infected Cats.- Frequencies of SARS-CoV-2 Spike Protein-Specific Memory B Cells in Human PBMCs, Quantified by ELISPOT Assay.- Monitoring Memory B Cells by Next Generation ImmunoSpot® Provides Insights into Humoral Immunity that Measurements of Circulating Antibodies Do Not Reveal.- Tracking Circulating HLA-Specific IgG-Producing Memory B Cells with the B Cell ImmunoSpot Assay.- Assessing the Affinity Spectrum of the Antigen-Specific B Cell Repertoire via ImmunoSpot®.- Using PBMCs in a Multiplex FluoroSpot Assay for Detection of Innate Immune Response Modulating Impurities (IIRMIs).- Four-Color ImmunoSpot® Assays Requiring Only 1-3 mL of Blood Permit Precise Frequency Measurements of Antigen-Specific B Cells Secreting Immunoglobulins of All Four Classes and Subclasses.- High-Plex Elispot: FOLISPOT Based on Fluorescence Detection and DNA Complementary Pairing.-  Triple-Color FluoroSpot Analysis of Polyfunctional Antigen-Specific T Cells by Quantification of Spot-Forming Units and Relative Spot Volumes.- Performance and Stability of New Class of Fetal Bovine Sera (FBS) and Its Lyophilized Form in ELISpot and FluoroSpot Assays: Applications for Monitoring the Immune Response in Vaccine, and Cell and Gene Immunotherapy in Clinical Trials.