Circular RNAs

Purification of circular RNAs using poly(A) tailing followed by RNase R digestion.- State of the art circular RNA analytics using the circtools software suite.- Exploring circular RNA profile and expression in extracellular vesicles.- Sanger sequencing to determine the full-length sequence of circular RNAs.- Droplet digital PCR for the detection and quantification of bona fide circRNAs.- Targeted sequencing of circular RNAs for Illumina-based counting and Nanopore structure determination.- Nanopore mediated sequencing of circular RNA.- In vivo tissue-specific knockdown of circRNAs using shRNAs in Drosophila Melanogaster.- Functional circular RNA screening via RfxCas13d/BSJ-gRNA system.- Base-editor-mediated circRNA knockout by targeting predominantly back-splice sites.- Directed Circularization of a Short RNA.- Engineering Synthetic circRNAs for Efficient CNS Expression.- Functional investigation of in vitro transcribed circular RNA and formulation into Lipid nanoparticles via microfluidic mixing.- Characterising post-transcriptional modifications of circRNAs to investigate biogenesis and translation.- Native circular RNA pulldown method to simultaneously profile RNA and protein interactions.- Screening and characterization of functional circRNAs in neuronal cultures.