Rheumatoid Arthritis

Collagen-induced arthritis models.- Human xenograft model.- Scaffolded chondrogenic spheroid-engrafted model.- Denervation-induced sarcopenia model.- Long-term constant subcutaneous drug administration.- Clinical Scoring of Disease Activity in Animal Models.- Histological analyses of arthritic joints in collagen-induced arthritis model mice.- Preparation of Joint Extracts.- Production of immunizing antigen proteoliposome using cell-free protein synthesis system.- Re-construction of protein/liposome complex.- Production of neutralizing antibody.- Autoantibody profiling using human autoantigen protein array and AlphaScreen.- Generation of Specific Aptamers.- Detailed Protocol for Predicting 3D Structure of DNA Aptamers and Performing In Silico Docking Calculations.- RNA interference ex vivo.- Lentiviral-mediated systemic RNA interference in vivo.- Lentiviral production platform.- Mesenchymal stem cell engineering.- Screening of Ca2+ influx in lymphocytes.- Single-cell Ca2+ imaging.- Electrophysiological methods to measure Ca2+ current.- Evaluation of mitochondrial respiratory function in murine splenocytes.- The Functional Assessment of T Cells.- Release of antibodies and cytokines from B cells.- Evaluation of autoreactive responses.- Macrophage polarization and osteoclast differentiation.- Scanning electron microscopic analysis of the bone-resorption activity in mature osteoclasts.- Animal Models of Vasculitis.- Evaluation of skin damage under UV exposure.- Bulk RNA-seq assessment of murine spleen  using a portable MinION sequencing device.- Institutional review board considerations for clinical trials.- Design an intervention study.- Assessment of disease activity, structural damage, and function in rheumatoid arthritis.- Assessment of musculoskeletal ultrasound of rheumatoid arthritis.- 16S rRNA gene amplicon analysis of human gut microbiota.