Endocannabinoid Signaling

​Need for Methods to Investigate Endocannabinoid Signaling.- Extraction and Simultaneous Quantification of Endocannabinoids and Endocannabinoid-like Lipids in Biological Tissues.- Measuring the Content of Endocannabinoid-like Compounds in Biological Fluids: A Critical Overview of Sample Preparation Methodologies.- LC-MS/MS Analysis of AEA and 2-AG.- Analysis of Omega-3 Fatty Acid Derived N-Acylethanolamines in Biological Matrices.- Endocannabinoid-Binding Receptors as Drug Targets.- Assay of CB1 Receptor Binding.- The Displacement Binding Assay Using Human Cannabinoid CB2 Receptor-Transfected Cells.- Fluorescence-based Assay for TRPV1 Channels.- DNA-Protein-Interaction (DPI)-ELISA Assay for PPAR-γ Receptor Binding.- Scintillation Proximity Assay (SPA)-Based Radioligand Binding for PPARα, PPARγ and PPARδ Receptors.- A Homogeneous Time-Resolved Fluorescence Resonance Energy Transfer (TR-FRET) Cofactor Recruitment Assay for PPARα andPPARγ.- The Cyclic AMP Assay Using Human Cannabinoid CB2 Receptor-Transfected Cells.- Assay of GTPγS Binding in Autoradiography.- Cellular Assay to Study β-Arrestin Recruitment by the Cannabinoid Receptors 1 and 2.- Endocannabinoid Metabolism and Transport as Drug Targets.- Assay of NAT Activity.- Radiometric Assay of NAPE-PLD activity.- A Fluorescence-based NAPE-PLD Activity Assay.- Radiometric Assay of FAAH Activity.- Fluorimetric Assay of FAAH activity.- Assay of NAAA Activity.- Assay of DAGLα/β Activity.- Assay of Monoacylglycerol Lipase Activity.- Radiometric Assay of ABHD2 Activity.- Oxygenation of Anandamide by Lipoxygenases.- Assay of Endocannabinoid Oxidation by Cytochrome P450.- Assays of Endocannabinoid Uptake.- Assessing Gene Expression of the Endocannabinoid System Components by Real-Time Quantitative Reverse Transcription Polymerase Chain Reaction.- Bioinformatics of the Endocannabinoid System: Study of DNA methylation at Rat Cnr1 gene promoter.- DNA Methylation Analysis of Cnr1 Gene Promoter.- Western Blotting of the Endocannabinoid System.- Quantitation of Plasma Membrane (G Protein-Coupled) Receptor Trafficking in Cultured Cells.- Assessing CB1 Expression in the Brain by Immunohistochemical Methods: Light, Confocal and Electron Microscopy.- Measuring Endocannabinoid System Interaction with Biomembranes.- STORM Super-Resolution Imaging of CB1 Receptors in Tissue Preparations.- Visualization of Endocannabinoids in the Cell.- Electrophysiology of Endocannabinoid Signalling.- Machine Learning and Computational Chemistry for the Endocannabinoid System.- A Virtual Reverse Screening Approach to Target Type 2 Cannabinoid Receptor.