Reverse Genetics of RNA Viruses

Methods and Protocols

Buch | Hardcover

272 Seiten

2023 | 2nd ed. 2024
Springer-Verlag New York Inc.
978-1-0716-3532-2 (ISBN)

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This second edition volume expands on the previous edition with new and updated chapters that highlight the latest methods and approaches for the manipulation of RNA viruses. The chapters in this book explore the fundamental role in studying RNA viruses; identifying markers of host range, disease, and transmission; and aid readers with the further development of in silico computational biology tools and artificial intelligence algorithms that can help predict the emergence of certain pathogens. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and comprehensive, Reverse Genetics of RNA Viruses: Methods and Protocols, Second Edition is a valuable resource for researchers who are interested in learning more about this developing field.

Reverse Genetics Systems for Filoviruses.- Reverse Genetics Systems for the de novo Rescue of Diverse Members of Paramyxoviridae.- Rescue of Recombinant Newcastle Disease Virus Expressing Heterologous Genes.- Use of Reverse Genetics for the Generation of Recombinant Influenza Viruses Carrying Nanoluciferase.- Reverse Genetics of Bat Influenza A Viruses.- Rescue of Infectious Salmon Anemia Virus (ISAV) from Cloned cDNA.- Reverse Genetics System for Rift Valley Fever Virus.- Plasmid-Based Lassa Virus Reverse Genetics.- Bacterial Artificial Chromosome Reverse Genetics Approaches for SARS-CoV-2.- Construction of Infectious Clones for Human Enteroviruses.- Reverse genetics of Hepatitis C Virus using an RNA Polymerase I-Mediated Transcription.- Reverse Genetics of Zika Virus using a Bacterial Artificial Chromosome.- A Stable Reverse Genetics System of Zika Virus Based on a Self-Splicing Group II Intron.- Reverse Genetics of Dengue Virus.- Recovery of Recombinant Rotaviruses by Reverse Genetics.

Erscheinungsdatum	13.12.2023
Reihe/Serie	Methods in Molecular Biology
Zusatzinfo	40 Illustrations, color; 5 Illustrations, black and white; X, 272 p. 45 illus., 40 illus. in color.
Verlagsort	New York, NY
Sprache	englisch
Maße	178 x 254 mm
Themenwelt	Medizin / Pharmazie ► Medizinische Fachgebiete ► Mikrobiologie / Infektologie / Reisemedizin
	Studium ► 2. Studienabschnitt (Klinik) ► Humangenetik
	Naturwissenschaften ► Biologie ► Genetik / Molekularbiologie
	Naturwissenschaften ► Biologie ► Mikrobiologie / Immunologie
Schlagworte	Genetic sequences of viruses • Nucleic acid engineering • Phenotypic effects • RG methods • Viral genetics
ISBN-10	1-0716-3532-8 / 1071635328
ISBN-13	978-1-0716-3532-2 / 9781071635322
Zustand	Neuware