Cancer Drug Resistance -

Cancer Drug Resistance

Methods and Protocols

Marta Baiocchi (Herausgeber)

Buch | Softcover
242 Seiten
2023 | 1st ed. 2022
Springer-Verlag New York Inc.
978-1-0716-2515-6 (ISBN)
139,09 inkl. MwSt
This volume discusses the latest techniques used to identify cancer drug resistance determinants at the molecular, cellular, and functional levels. Chapters in this book cover up-to-date topics including tumor-microenvironment cell co-culture methods and microfluidics systems; workflows for functional assessment of drug resistance in vitro and in vivo; quantitative techniques for identifying quiescent blood-flow circulating cells; and single-cell characterization methods, such as mass cytometry. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and practical, Cancer Drug Resistance: Methods and Protocols is a valuable resource for all scientists and researchers who are looking to learn more about the latest developments in understanding and overcoming anticancer drug resistance.  

Preface..Table of Contents...Contributing Authors...

1.A 3D Biomimetic System for Testing Anticancer Drugs SensitivityMiodrag Dragoj, Jasmina Stojkovska, Sofija Jovanovic Stojanov, Bojana Obradovic, and Milica Pesic
2.Patient-Derived Breast Cancer Tissue Cultures for Anti-Endocrine Drug AssaysGiacomo Domenici, Gonçalo Trindade, Marta F. Estrada, Ana Luísa Cartaxo, Paula M. Alves, Saudade André, and Catarina Brito
3.Co-Culture of Two Different Cell Lines in a Two-Layer Microfluidic DeviceSharif M. Rahman, Elizabeth C. Martin, and Adam T. Melvin
4.Generation and Culture of Organotypic Breast Carcinoma Spheroids for the Study of Drug Response in a 3D Microfluidic DeviceHamidreza Aboulkheyr Es, Amir Reza Aref, and Majid Ebrahimi Warkiani
5.A Methodological Workflow to Analyze Synthetic Lethality and Drug Synergism in Cancer Cells Johann Gout, Elodie Roger, Alexander Kleger, and Lukas Perkhofer
6.Establishment of In Vivo Acquired Resistance to Chemotherapy via Individual Dose Escalation Treatment RegimeKaren T. Barker and Orli Yogev
7.A Label Retaining System to Capture Slow Cycling Cancer CellsIsabel Puig and Héctor G. Palmer
8.A Fast and Furious Liquid Biopsy Assay to Monitor Targeted Therapy ResistanceChiara Nicolazzo and Paola Gazzaniga
9.Assessment of Tumor Heterogeneity in High-Grade Serous Ovarian Cancer: Mass Cytometry to Understand the Complex Tumor BiologyLuca Pasquini, Roberta Riccioni, and Eleonora Petrucci
10.Analysis of Transcriptomic Data Generated from Drug Treated Cancer Cell LineSwarnima Kushwaha, Sudeshna Mukherjee, Rajdeep Chowdhury, and Shibasish Chowdhury
11.Simultaneous Mapping of Enhancers and Enhancer Rearrangements with Paired-End H3K27ac ChIP-seqIsrael Antman, Ester Davis, Shahd Abu-Kamel, Merav Hecht, and Yotam Drier
12.Identification of Drug Resistance Mechanisms using Genome-Wide CRISPR-Cas9 ScreensGraham MacLeod, Nishani Rajakulendran, and Stephane Angers
13.RNA Sequencing Data Analysis on the Maser Platform and the Tag-Count Comparison-Graphical User InterfaceKenta Ujifuku, Yoichi Morofuji, and Hiroshi Masumoto
14.Dissecting the Epigenomic Driving Drug Resistance by ATAC-SeqFrancesca de Nicola, Giacomo Corleone, and Frauke Goeman
15.Polygenomic Interrogation of Drug Resistance GenesRobert J. Autry
16.Analysis of ER-phagy in Cancer Drug ResistanceSandhya Chipurupalli, Vincenzo Desiderio, and Nirmal Robinson
17.Drug Target Prediction using Context-Specific Metabolic Models Reconstructed from rFastcormicsTamara Bintener, Maria Pires Pacheco, Ali Kishk, Jeff Didier, and Thomas Sauter
Subject Index List...

Erscheinungsdatum
Reihe/Serie Methods in Molecular Biology
Zusatzinfo 56 Illustrations, color; 19 Illustrations, black and white; XIII, 242 p. 75 illus., 56 illus. in color.
Verlagsort New York, NY
Sprache englisch
Maße 178 x 254 mm
Themenwelt Medizin / Pharmazie Medizinische Fachgebiete Onkologie
Medizin / Pharmazie Medizinische Fachgebiete Pharmakologie / Pharmakotherapie
Medizin / Pharmazie Studium
Schlagworte Determinants of resistance • Drug design and targeting • Drug resistant cells • early detection • Molecular analysis
ISBN-10 1-0716-2515-2 / 1071625152
ISBN-13 978-1-0716-2515-6 / 9781071625156
Zustand Neuware
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