The Olfactory System -

The Olfactory System

Buch | Hardcover
224 Seiten
2023 | 1st ed. 2023
Springer-Verlag New York Inc.
978-1-0716-3424-0 (ISBN)
171,19 inkl. MwSt
lt;p>This volume discusses the latest approaches used to investigate molecular biology and physiology in the peripheral and central olfactory system. Understanding both normal function and pathobiology provides readers with insight into understanding human olfactory disorders. The chapters in this book cover topics such as techniques for manipulating and measuring olfactory cilia function; approaches for investigating spatial RNA expression in olfactory mucosa; the use of chromatin immunoprecipitation with olfactory neurons; in vivo methods to label and identify activated olfactory neurons; defining organization in the olfactory bulbs and cortex; and the use of optical recording in the mouse olfactory bulb for acute or chronic experiments. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Thorough and cutting-edge, The Olfactory System: Methods and Protocols is a valuable resource for all researchers who are interested in learning more about this important and developing field.


lt;p>Preface...
Table of Contents...
Contributing Authors...

1.Visualizing and Manipulating Olfactory Cilia through Viral Delivery Coupled with En Face Imaging of Intact OE
Julien C. Habif, Chao Xie, and Jeffrey R. Martens

2.Genome-Wide RNA Tomography in the Mouse Whole Olfactory Mucosa
Eman Abou Moussa, Melanie Makhlouf, Lisa S. Mathew, and Luis R. Saraiva

3.Optical Activation of Photoswitchable TRPC Ligands in the Mammalian Olfactory System Using Laser Scanning Confocal Microscopy
Navin K. Ojha, Frank Zufall, and Trese Leinders-Zufall

4.Intranasal Pressure Recording for Monitoring Mouse Respiration
Emma Janke, Janardhan P. Bhattarai, and Minghong Ma

5.Identification of Immune Cells in Murine Olfactory Mucosa
Sebastian A. Wellford and E. Ashley Moseman

6.Chromatin Immunoprecipitation from Formaldehyde Cross-Linked Olfactory Sensory Neurons
Jerome Kahiapo and Kevin Monahan

7.Activity-Dependent Labeling of Olfactory Sensory Neurons using RNA Fluorescence In Situ Hybridization followed by Phospho-S6 Immunofluorescence
Maira Harume Nagai and Hiroaki Matsunami

8.Measuring Cell Surface Expression of Odorant Receptors Via Flow Cytometry 
Jeevan Tewari and Hiroaki Matsunami

9.Dissociation of Mouse Olfactory Mucosae for Fluorescence-Activated Cell Sorting of Olfactory Sensory Neurons
Qiang Wang, Tomoko Sengoku, William B. Titlow, Jennifer L. Strange, and Timothy S. McClintock

10.Preparation of Human Olfactory Epithelial Biopsies for Downstream Analysis
Rhea Choi, Tiffany Ko, John B. Finlay, Ralph Abi Hachem, David Jang, and Bradley J. Goldstein

11.Cranial Window for Acute and Chronic Optical Access to Record Neuronal Network Dynamics in the Olfactory Bulb
Marco Brondi and Claudia Lodovichi

12.Target-Captured mRNA from Murine Olfactory Bulb
Kevin W. Zhu and Hiroaki Matsunami

13.Identification and Localization of Cell Types in the Mouse Olfactory Bulb using Slide-SeqV2
Ai Fang, Kaitlyn Petentler, Andrew Price, Seth Malloy, Cindy Maddera, Jonathan Russel, McKenzie Treese, Hua Li, Yongfu Wang, Sean McKinney, Anoja Perera, and C. Ron Yu

14. Dense and Sparse Labelling of Mitral Cells by Oral and Intraperitoneal Routes of Tamoxifen Administration
Xiaochen Fu, Yu-Pei Huang, Sander Lindeman, Adam Mago, and Izumi Fukunaga

15.Techniques in Staining of Rodent and Human Olfactory Tissue
Hironobu Nishijima and Eric H. Holbrook

16.Electrophysiological Recordings from Identified Cell-Types in the Olfactory Cortex of Awake Mice
Kevin A. Bolding and Kevin M. Franks

Subject Index List...

Erscheinungsdatum
Reihe/Serie Methods in Molecular Biology
Zusatzinfo 43 Illustrations, color; 3 Illustrations, black and white; XI, 224 p. 46 illus., 43 illus. in color.
Verlagsort New York, NY
Sprache englisch
Maße 178 x 254 mm
Themenwelt Medizin / Pharmazie Studium
Schlagworte Drosophila • Immune Function • Plethysmography • PS6-IPseq • Translational Medicine
ISBN-10 1-0716-3424-0 / 1071634240
ISBN-13 978-1-0716-3424-0 / 9781071634240
Zustand Neuware
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