Mammary Stem Cells

Protocol for studying embryonic mammary gland branching morphogenesis ex vivo.- Multidimensional fluorescence imaging of embryonic and postnatal mammary gland development.- Single-cell transcriptomic and epigenetic analyses of mouse mammary development starting with the embryo.- Computational methods to identify cell fate determinants, identity transcription factors and niche induced signaling pathways for stem cell research.- Differential proteomic analysis of complex mixtures by label free nLC MS/MS.- Orthotopic transplantation of mouse mammary epithelial cells.- Lineage tracing methods to study mammary epithelial hierarchies in vivo.- Lentiviral transduction of mammary epithelial cells.- Modification of Single Cells within Mouse Mammary Gland Derived Acini via Viral Transduction.- Surgical Procedure for Implantation of Human Tumor Tissue into the Epithelium-free Mammary Fat Pad of Immunocompromised Mice to Generate Patient-Derived Xenografts (PDX).- The Chicken Embryo Chorioallantoic Membrane (CAM); a Versatile Tool for the Study of Patient-Derived Xenografts.- Intraductal injections into the mouse mammary gland.- Modelling breast cancer in organoid and intraductal models.- Single organoid droplet-based staining method for high-end 3D imaging of mammary organoids.- Divide and Conquer: Isolating Cell Populations to Investigate how Breast Cancer Risk Factors Alter the Breast Microenvironment.- An organotypic assay to study epithelial-fibroblast interactions in human breast.- Patient-derived explant cultures of normal and tumour human breast tissue.- Negative-Selection Enrichment of Circulating Tumor Cells from Peripheral Blood Using the Microfluidic CTC-iChip.- Measuring Mechanical Properties of Breast Cancer Cells with Atomic Force Microscopy.- Breast cancer stem cells: a clinician´s view.