The Immune Synapse

Measuring the Co-Localization and Dynamics of Mobile Proteins in Live Cells Undergoing Signaling Responses.- Fluorescence-Based Measurements of Two-Dimensional Affinity in Membrane Interfaces.- Surfaces for Study of Receptor Dynamics on T Cells.- Molecular Dynamics Simulations of Immune Receptors and Ligands.- Investigating Diffusion Dynamics and Interactions with Scanning Fluorescence Correlation Spectroscopy (sFCS).- Combined FRET-FLIM and NAD(P)H FLIM to Analyze B Cell Receptor Signaling Induced Metabolic Activity of Germinal Center B Cells In Vivo.- Measurement of Molecular Height Using Cell Surface Optical Profilometry (CSOP).- Observing Membrane and Cell Adhesion via Reflection Interference Contrast Microscopy (RICM).- En-Face-Imaging of T Cell-Dendritic Cell Immunological Synapses.- High and Super Resolution Imaging of Cell-Cell Interfaces.- Separation of Single Core and Multicore Lytic Granules by Subcellular Fractionation and Immuno-Isolation.- Microvillar Cartography: A Super-Resolution Single-Molecule Imaging Method to Map the Positions of Membrane Proteins with Respect to Cellular Surface Topography.- T Cell Immunological Synaptosomes: Definition and Isolation.- Dynamics of Immune Cell Microvilli.- Visualization of Myddosome Assembly in Live Cells.- Detection of Telomere Transfer at Immunological Synapse.- Bottom-Up Assembly of Bio-Inspired, Fully Synthetic Extracellular Vesicles.- A DNA Origami-Based Biointerface to Interrogate the Spatial Requirements for Sensitized T-Cell Antigen Recognition.- Leveraging DNA Origami to Study Phagocytosis.- Fabrication of Nanoscale Arrays to Study the Effect of Ligand Arrangement on Inhibitory Signaling in NK Cells.- Measurement of Forces for Trans-Endocytosis at Dorsal and Ventral Sides of the Cell.- Functionalized Lipid Droplets and Microfluidics Approach to Study Immune Cell Polarity In Vitro.- Quantifying Immune Cell Force Generation Using Traction Force Microscopy.- Characterizing Biophysical Parameters of Single TCR-pMHC Interactions Using Optical Tweezers.- Isolation of the B Cell Immune Synapse for Proteomic Analysis.- Analyzing Single Cell Secretions by ‘Shadow Imaging’.- Exploiting the RUSH System to Study Lytic Granule Biogenesis in Cytotoxic T Lymphocytes.- Interactions of Tissue Resident T Cells.- Live Imaging of CAR T Cell Ca2+ Signals in Tumor Slices Using Confocal Microscopy.- Measuring CTL Lytic Granule Secretion and Target Cell Membrane Repair by Fluorescent Lipophilic Dye Uptake at the Lytic Synapse.- In Vitro Generation of Human Tolerogenic Monocyte-Derived Dendritic Cells.- Methods of Machine Learning-Based Chimeric Antigen Receptor Immunological Synapse Quality Quantification.- Imaging CAR-T Synapse as a Quality Control for CAR Engineering.