Developmental, Physiological, and Functional Neurobiology of the Inner Ear

Pou4f3DTR Mice Enable Selective and Timed Ablation of Hair Cells in Post-Natal Mice.- Cochlear Explant Cultures: Creation and Application.- Immunohistochemistry and In Situ mRNA Detection using Inner Ear Vibratome Sections.- Genetic Manipulation of the Embryonic Chicken Inner Ear.- Molecular Tools to Study Regeneration of the Avian Cochlea and Utricle.- An Efficient Method to Detect Messenger RNA (mRNA) in the Inner Ear by RNAscope In Situ Hybridization.- A Manual Technique for Isolation and Single-Cell RNA Sequencing Analysis of Cochlear Hair Cells and Supporting Cells.- Ribosomal Pulldown Assays and Their Use to Analyze Gene Expression in Multiple Inner Ear Cell Types.- In Vivo Analysis of Hair Cell Sensory Organs in Zebrafish: From Morphology to Function.- Electrophysiological Recordings of Voltage Dependent and Mechanosensitive Currents in Sensory Hair Cell of the Auditory and Vestibular Organs of the Mouse.- Biophysical Recording from Adult Hair Cell.- Endocochlear Potential Measures, Local Drug Application, and Perilymph Sampling in the Mouse Inner Ear.- In Vivo Whole-Cell Recording in the Gerbil Cochlear Nucleus.- Measurement of Human Cochlear and Auditory Nerve Potentials.- Strategies for Identification of Medial Olivocochlear Neurons for Patch-Clamp Studies of Synaptic Function using Electrical Stimulation and Optogenetics.- Auditory Brainstem Response (ABR) Measurements in Small Mammals.- Behavioral Models Loudness, Hyperacusis, and Sound Avoidance.