Inclusion Bodies
Springer-Verlag New York Inc.
978-1-0716-2929-1 (ISBN)
Authoritative and practical, Inclusion Bodies: Methods and Protocols serves as an ideal resource for facilitating diverse aspects of IB processing.
Inclusion Bodies: Status Quo and Perspectives.- Thermoinducible E. coli for Recombinant Protein Production in Inclusion Bodies.- High-Throughput Expression of Inclusion Bodies on an Automated Platform.- Design, Production, and Characterization of Catalytically-Active Inclusion Bodies.- Bidirectional Promoter Libraries Enable the Balanced Co-Expression of Two Target Genes in E. coli.- Inclusion Body Production in Fed-Batch and Continuous Cultivation.- Avoiding the All-or-None Response in Gene Expression during E. coli Continuous Cultivation Based on the On-Line Monitoring of Cell Phenotypic Switching Dynamics.- Optimization of Inclusion Body Formation and Purification in Multi-Well Plates.- High Pressure Homogenization for Inclusion Body Isolation.- Development of Solubilization and Refolding Buffers.- Unit Operation-Spanning Investigation of the Redox System.- Using High Pressure and Alkaline pH for Refolding.- Intensification of Inclusion Body Processing viaTemperature-Based Refolding.- Refolding of Proteins Expressed as Inclusion Bodies in E. coli.- Infrared Spectroscopy for Structure Analysis of Protein Inclusion Bodies.- The Purification of Heme Peroxidases from Escherichia coli Inclusion Bodies: A Success Story Shown by the Example of Horseradish Peroxidase.- Production of α-Synuclein Fibrillar Specific scFv from Inclusion Bodies.- Method for Inclusion Bodies Production via E. coli Host System: rGCSF as Model Biotherapeutic Protein.- Antimicrobial Applications of Inclusion Bodies.
Erscheinungsdatum | 24.01.2023 |
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Reihe/Serie | Methods in Molecular Biology ; 2617 |
Zusatzinfo | 47 Illustrations, color; 8 Illustrations, black and white; XI, 272 p. 55 illus., 47 illus. in color. |
Verlagsort | New York, NY |
Sprache | englisch |
Maße | 178 x 254 mm |
Themenwelt | Medizin / Pharmazie ► Pharmazie |
Naturwissenschaften ► Biologie ► Biochemie | |
Naturwissenschaften ► Biologie ► Genetik / Molekularbiologie | |
Naturwissenschaften ► Chemie | |
Technik ► Maschinenbau | |
Technik ► Umwelttechnik / Biotechnologie | |
Schlagworte | biopharmaceuticals • Biosimilars • Escherichia coli • Fed-batch cultivations • IB processing • recombinant proteins |
ISBN-10 | 1-0716-2929-8 / 1071629298 |
ISBN-13 | 978-1-0716-2929-1 / 9781071629291 |
Zustand | Neuware |
Informationen gemäß Produktsicherheitsverordnung (GPSR) | |
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