Basic and Advanced Laboratory Techniques in Histopathology and Cytology

Pranab Dey is professor in department of cytology and gynecologic pathology at Post Graduate Institute of Medical Education and Research (PGIMER), Chandigarh, India. Professor Dey completed M.D. (Pathology) from PGIMER and FRCPath (Cytopathology) from Royal College of Pathologist, London, UK. Professor Dey has conducted many research projects and has pioneered works on DNA flow cytometry, image morphometry, mono-layered cytology and cytomorphologic findings of various lesions on cytology smears. Professor Dey has more than 35 years of experience in reporting, teaching and research as a consultant. He is a well published author in pathology and a member of various societies. He has more than 450 research paper publications in peer-reviewed journals. He has authored 12 books in cytology and gynecologic pathology. Dr Dey is included in the list of top 2% scientists in the world.

Chapter 1: Fixation of histology samples: Principles, methods and types of fixatives.- Chapter 2:  Processing of tissue in the histopathology laboratory.- Chapter 3: Embedding of tissue in histopathology.- Chapter 4: Decalcification of bony and hard tissue for histopathology processing.- Chapter 5: Tissue Microtomy: principle and procedure.- Chapter 6: Frozen section: Principle and procedure.- Chapter 7: Staining principle and general procedure of staining of the tissue.- Chapter 8: Haematoxylin and Eosin stain of the tissue section.- Chapter 9: Special Stains for the carbohydrate, protein, lipid, nucleic acid and pigments.- Chapter 10: Connective tissue stain: Principle and procedure.- Chapter 11: Amyloid staining.- Chapter 12: Stains for the Microbial Organisms.-  Chapter 13: Cytology sample procurement, fixation and processing.- Chapter 14: Routine staining in cytology laboratory.- Chapter 15: The basic technique of fine needle aspiration cytology.- Chapter 16: Immunocytochemistry in histology and cytology.- Chapter 17: Flow cytometry: Basic principles, procedure and applications in pathology.- Chapter 18: Digital pathology.- Chapter 19:  Automation in the laboratory and Liquid-based cytology.- Chapter 20: Polymerase chain reaction: Principle, technique and applications in pathology.- Chapter 21: Fluorescent in situ hybridisation techniques in pathology: Principle, technique and applications.- Chapter 22: Tissue microarray in pathology: Principal, technique and applications.- Chapter 23: Sanger sequencing and next-generation gene sequencing: Basic principles and applications in pathology.- Chapter 24: Liquid biopsy: Basic principles, techniques and applications.- Chapter 25: Artificial neural network in Pathology: Basic principles and applications.- Chapter 26: Compound Light microscope and other different microscopes.- Chapter 27: Fluorescence microscope, confocal microscope and other advanced microscopes: Basic principles and applications in pathology.- Chapter 28: Electron microscopy: Principle, components, optics and specimen processing.- Chapter 29: Quality control and laboratory organization.- Chapter 30: Laboratory safety and laboratory waste disposal.- Multiple choice questions for the self-assessment.