T-Cell Development

A beginner’s guide to T cell development.- Revelations in thymic epithelial cell biology and heterogeneity from single cell RNA sequencing and lineage tracing methodologies.- Early development of innate lymphoid cells.- Development of gd T cells, Soldiers on the Front Lines of Immune Battles.- Principles of Advanced Flow Cytometry: A Practical Guide.- Genetic strategies to study T cell development.- A simplified approach to evaluating T cell development by flow cytometry.- Purification of thymocyte and T cell subsets.- Generation of bone marrow chimeras.- Generation of murine T cell effector populations in vitro.- Large-scale isolation of mouse thymic epithelial cells.- Generation of retrogenic mice to investigate T cell developmentPurification of bone marrow precursors to T cells and ILCs.- Studying T cell Development in Neonatal and Adult Thymic Slices.- Induction of Human T Cell Development In Vitro with OP9-DL4-7FS Cells Expressing Human Cytokines.- Methods for Study of Mouse T Cell Receptor a and b Gene Rearrangements.- Ultrasound guided intra-thymic cell injection.- Fetal thymic organ culture and negative selection.- In Vitro Analysis of Thymocyte Signaling.- Identification and purification of human T cell precursors.- In vitro model systems to study human T cell development.- Zebrafish: A Tractable Model for Analysis of T cell Development.