Long Non-Coding RNAs in Cancer

 LncRNAs and Available Databases.- LncRNA as Cancer Biomarkers.- Bioinformatics Pipeline to Analyze lncRNA Arrays.- Boinformatic Pipelines to Analyze lncRNAs RNAseq Data.- Single Cell RNAseq Analysis of lncRNAs.- Measuring lncRNA Expression by Real Time PCR.- Quantitation of Long Non-coding RNA using Digital PCR.- Detection of lncRNA by LNA-based In Situ Hybridization in Paraffin Embedded Cancer Cell Spheroids.- In vitro Silencing of lncRNA Expression using siRNAs.- In vitro silencing of lncRNAs using LNA gapmeRs.- Methods used to make Lipid Nanoparticles to deliver LNA Gapmers against lncRNAs into Acute Myeloid Leukemia (AML) Blasts.- CRISPR/Cas9 to Silence Long Non-coding RNAs.- CRISPR interference (CRISPRi) and CRISPR activation (CRISPRa) to explore the oncogenic lncRNA network.- In vivo Silencing/Overexpression of lncRNAs by CRISPR/Cas System.- Experimental Validation of the Non-Coding Potential for lncRNA.- Identification of lncRNA-protein interactions by CLIP and RNA pull-down assays.- Empirical Validation of Overlapping Virus lncRNAs and Coding Transcripts by Northern Blot.- Phospho-RNAseq profiling of extracellular mRNAs and lncRNAs.- Detection of Circulating RNA using Nanopore Sequencing.- LncRNA Quantification from Extracellular Vesicles Isolated from Blood Plasma or Conditioned Media.- The use of circRNAs as Biomarkers of Cancer.- Bioinformatic Analysis of Circular RNA Expression.- Study of circular RNAs (circRNAs) expression.