Tumor Angiogenesis Assays

Discovery and development of tumor angiogenesis assays.- The role of the stroma in tumor angiogenesis and progression: the fundamental contribution of Pietro M. Gullino.- Methods for Isolation of Tumor Associated Endothelial Cells for Surface Protein Analysis and Sorting by Flow cytometry.- Perfusion based fluorescent dye labeling to sort cancer cells based on their distance from blood vessels.- Visualizing the interactions that haping the imaging of microenvironment in cancer diseases.- Quantification of tumor and angiogenic-related markers in ovarian cancer models by a digital pathology approach.- Monitoring angiotropic extravascular migratory metastasis in vitro.- Double immunohistochemical staining on formalin-fixed paraffin-embedded tissue samples to study vascular co-option.- Development of Experimental Three-Dimensional Tumor Models to Study Glioblastoma Cancer Stem Cells and Tumor Microenvironment.- IKOSA® CAM Assay application to quantify blood vessels on chick chorioallantoic membrane (CAM).- Application of laser speckle contrast imaging (LSCI) for the angiogenesis measurement of tumors in the chorioallantoic membrane (CAM) model.- The chick CAM as an in vivo system to study stem cell activity.- Detection of possible symmetries in vascular networks by computer assisted image analysis.- Alternative in vivo models to study teratoma.- How to generate a vascular-labeled transgenic zebrafish model to study tumor angiogenesis and extravasation.- 3D human tumor tissues cultured in dynamic conditions as alternative in vitro disease models.