Handbook of Immunohistochemistry and in situ Hybridization of Human Carcinomas
Academic Press Inc (Verlag)
978-0-12-088404-9 (ISBN)
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Immunohistochemistry is the use of specific antibodies to stain particular molecular species in situ. This technique has allowed the identification of many more cell types than could be visualized by classical histology, particularly in the immune system and among the scattered hormone-secreting cells of the endocrine system, and has the potential to improve diagnosis, prognosis and therapeutic options of cancer. This book discusses all aspects of immunohistochemistry and in situ hybridization technologies and the important role they play in reaching a cancer diagnosis. It provides step-by-step instructions on the methods of additional molecular technologies such as DNA microarrays, and microdissection, along with the benefits and limitations of each method. The topics of region-specific gene expression, its role in cancer development and the techniques that assist in the understanding of the molecular basis of disease are relevant and necessary in science today.
Dr. Hayat has published extensively in the fields of microscopy, cytology, immunohistochemistry, immunocytochemistry, and antigen retrieval methods. He is Distinguished Professor, Department of Biological Sciences, Kean University, Union, New Jersey, USA.
Chapter 1: Quality Assurance in Immunohistochemistry
Chapter 2: Methodology of Microarray Data Analysis
Chapter 3: Microarray-Based Gene Expression Analysis of Defined Tumor Areas
Chapter 4: Tissue Microarray and Quantitative Fluorescence Image Analysis in Tumor Biomarker Analysis
Chapter 5: Identification of Differentially Expressed Genes Using Rapid Subtraction Hybridization: Detailed Methodology for Performing Rapid Subtraction Hybridization
Chapter 6: Noncontact Laser Microdissection and Pressure Catapulting: A Basic Tool in Genomics, Transcriptomics, and Proteomics
Chapter 7: DNA Methylation Analysis of Human Cancer
Chapter 8: Antiepidermal Growth Factor Receptor Antibody: Immunohistochemistry
Chapter 9: PTEN and Cancer
Chapter 10: The Role of p53 and p73 Genes in Tumor Formation
Chapter 11: Analysis of Centrosome Amplification in Cancer
Chapter 1: Liver Carcinoma
Chapter 2: Mechanisms of Human Hepatocarcinogenesis: An Overview
Chapter 3: Genes Involved in Hepatocellular Carcinoma
Chapter 4: Role of p53 and ZBP-89 in Hepatocellular Carcinoma
Chapter 5: Role of Immunohistochemical Expression of PTEN in Hepatocellular Carcinoma
Chapter 6: The Potential Role of Bcl-2 and Protein Expression in Hepatocellular Carcinomas: A Clinicopathologic Study
Chapter 7: The Detection and Clinical Aspect of Immunohistochemical Analysis of Bcl-xL Protein in Hepatocellular Carcinoma
Chapter 8: Growth Arrest DNA Damage-Inducible Gene 45 ß in Human Hepatocellular Carcinoma
Chapter 9: Immunohistochemical Detection of DNA Topoisomerase IIa in Hepatocellular Carcinoma
Chapter 10: Role of Immunohistochemical Expression of DNA Methyltransferases in Hepatocellular Carcinoma
Chapter 11: Role of Hepatitis B Surface Antigen in Hepatocarcinogenesis
Chapter 12: Immunohistochemical Detection of Host Gene Products Up-Regulated by Hepatitis B Virus X Antigen and Their Putative Roles in Hepatocellular Carcinoma
Chapter 13: Distinction between Well-Differentiated Hepatocellular Carcinoma and Benign Hepatocellular Nodular Lesions, Using Fine-Needle Aspiration Biopsy
Chapter 14: Immunohistochemical Detection of EBAG9/RCAS1 Expression in Hepatocellular Carcinoma
Chapter 15: Hepatocellular Carcinoma of Differential Viral Origin: Analysis by Oligonucleotide Microarray
Chapter 1: Pancreatic Carcinoma: An Introduction
Chapter 2: Detection of Genomic Imbalances in Endocrine Pancreatic Tumors Using Comparative Genomic Hybridization
Chapter 3: Molecular Detection of Micrometastases in Pancreatic Cancer
Chapter 4: K-ras Protooncogene in Human Pancreatic Cancer
Chapter 5: Altered Expression of p27 Protein in Pancreatic Carcinoma
Chapter 6: The Role of DUSP6/MKP-3 in Pancreatic Carcinoma
Chapter 7: Expression of Carbohydrate Antigens in Pancreatic Cancer
Chapter 8: Role of Mucins in Pancreatic Carcinoma
Chapter 9: Immunohistochemical Detection of Cyclooxygenase-2 in Pancreatic Ductal Adenocarcinomas and Neuroendocrine Tumors
Chapter 10: Immunohistochemical Detection of Activated Stat3 Protein in Pancreatic Cancer
Chapter 11: The Role of Transcription Factor E2F-1 in Pancreatic Ductal
Chapter 12: Role of Immunohistochemical Expression of Chromogranins in Pancreatic Endocrine Tumors
Chapter 13: Role of Immunohistochemical Expression of Maspin in Pancreatic Carcinoma
Chapter 14: Role of Mast Cells in Pancreatic Carcinoma
Chapter 15: Endocrine Cells in Invasive Ductal Adenocarcinoma of the Pancreas: An Immunohistochemical Study
Chapter 16: Fine-Needle Aspiration Biopsy of Pancreatic Adenocarcinoma
Chapter 17: Immunohistochemical Expression of Mitogen-Activated Protein Kinase Kinase 2 in Pancreatic Cancer
Index
Erscheint lt. Verlag | 21.9.2005 |
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Verlagsort | San Diego |
Sprache | englisch |
Maße | 216 x 279 mm |
Gewicht | 1840 g |
Themenwelt | Medizin / Pharmazie ► Medizinische Fachgebiete ► Onkologie |
ISBN-10 | 0-12-088404-6 / 0120884046 |
ISBN-13 | 978-0-12-088404-9 / 9780120884049 |
Zustand | Neuware |
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