Gene Therapy of Cancer

Brief Introduction to Current Cancer Gene Therapy.- Minicircles for CAR T Cell Production by Sleeping Beauty Transposition - A Technological Overview.- Generation of CAR-T Cells with Sleeping Beauty Transposon Gene Transfer.- Generation of Redirected Engineered Human Chimeric Antigen Receptor (CAR) T cells.- TCR- and CAR-engineering of Primary Human T cells.- TCR Gene Therapy for Cancer.- Gene Gun Her2/neu DNA Vaccination: Evaluation of Vaccine Efficacy in a Syngeneic Her2/neu Mouse Tumor Model.- Construction of Minicircle Suicide Genes Coding for Ribosome-Inactivating Proteins.- Claudin-targeted Suicide Gene Therapy for Claudin-Overexpressing Tumor Cells by Using Modified Clostridium Perfringens Enterotoxin (CPE).- Bioengineered RNA Therapy in Patient-Derived Organoids and Xenograft Mouse Models.- Application of Transcription Factor Decoy Oligodeoxynucleotides (ODNs) for Cancer Therapy.- NK Cell Effector Functions and Bystander Tumor Cell Killing in Immunovirotherapy.- A qPCR-based Method for Quantification of Replication Competent Adenovirus (RCA) in Conditionally Replicating Oncolytic Adenoviruses.- Visualized Cancer Immunotherapy with Engineered Salmonella typhimurium.- CANCER Gene Therapy: Development and Production Of Lentiviral Vectors For Gene Therapy.- Capillary Gel Electrophoresis (CGE) for Quality Control of Plasmid DNA in Gene Therapy: quality control of 20-years stored GMP-grade Plasmid DNA.- Efficient Generation of Superior Dumbbell-Shaped Non-viral DNA Delivery Vectors Using 1-2-3 Gap-primer PCR.