African Swine Fever Virus

Working safely with African swine fever virus.- Necropsy procedures and evaluation of macroscopic lesions of pigs infected with African swine fever virus.- Preparation of immunofluorescently labelled tissue sections for imaging at low and high magnifications in the confocal microscope.- Primary macrophage culture from porcine blood and lungs.- Isolation of porcine bone marrow cells and generation of recombinant African swine fever viruses.- Laboratory diagnosis and quantification of African swine fever virus using real-time polymerase chain reaction.- Quantification of ASFV DNA and RNA in Ornithodoros soft ticks.- Genotyping of African swine fever virus.- African swine fever virus (ASFV) indirect ELISA test based on the use of the soluble cytoplasmic semi purified antigen (ASFV CP-Ag).- Indirect immunoperoxidase test (IPT) for detection of antibodies against African swine fever virus (ASFV) on African green monkey cell lines (Vero, MS).- African Swine Fever Virus Haemadosrption Inhibition Assay.- ELISpot assay for the detection of ASFV-specific interferon-gamma (IFN-γ) producing cells.- Purification of African swine fever virus.- African swine fever virus plaque assay and disinfectant testing.- Bioorthogonal labelling of African swine fever virus infected cells.- Whole genome sequencing of African swine fever virus.