Handbook of Free Radicals and Antioxidants in Biomedicine

Jaime Miquel, Ph.D. presently holds the position of Research Associate at the Linus Pauling Institute of Science and Medicine in Alicante. Alexandre T. Quintanilha, Ph.D., D.Sc., is Director of the Center for Atmosphere and Biospheric Effects of Technology and Assistant Head of the Applied Science Division at the Lawrence Berkeley Laboratory. Hans U. Weber, Ph.D., in 1987 joined the Medical Writing Group of Syntex Rsearch, Palo Alto, California.

Selected Models and Methods for Biological and Clinical Research The Carbon Tetrachloride Hepatoxicity Model: Free Radicals and Calcium Homeostasis. Models of Brain Lipoperoxidation: Inhibitory Effects of Mannitol, Vitamin E, and Glucocorticoid. Role of Oxidative Stress in Tumor Promotion. Effects of Vitamin C on Experimental Malignant Tumors. Fatty Acid Composition of Phospholipids in Heart Muscle Modified by Age, Diet, and Stress. Lesions of Aortic Intima Caused by Linoleic Acid Hydroperoxide. Peroxide-Induced Membrane Damage in Red Blood Cells. Oxidized Fatty Acids and Phosphatidic in Liposomes. Spectroscopic Techniques in the Study of Lipid Oxidation (except ESR Techniques). Chemiluminescence Methods for Assays of Lipoxygenase, 1,4-Pentadiene Fatty Acids, and Oxygen Concentration. Measurement of Intracellular Oxygen. Chemical Determination of Oxidation Damage to DNA. Elecron Spin Resonance in Biology. General Mechanisms of Spin Trapping in Vitro and in Vivo. Spin-Trapping Studies of Xenobiotic-Mediated Toxicity. Application of Electron Spin Resonance Techniques to the Detection of Free Radicals in Muscle Tissue. A Simple Fluorometric Assay for Lipid Peroxides in Blood Serum or Plasma. Chromogenic Determination of Lipid Hydroperoxides by Sesamol Dimer. Measurements of Individual Antioxidants and Radical-Trapping Activity. Analysis of Ascorbic Acid in Body Fluids and Tissues. Analysis of Tocopherol in Blood and Liver. Methods for the Analysis of Vitamin E in Animal Tissue. Determination of Reduced and Oxidized Ubiquinones. HPLC Analysis of Thiols Using Monobromobimane Fluorescent Labeling: Application to Radioprotective Drugs. Selenium in Human Urine: Determination and Clinical Implications. Determination of Glutathione Peroxidase. Convenient Assays for Superoxide Dismutase.