Engineering Natural Product Biosynthesis

A bioinformatics workflow for investigating fungal biosynthetic gene clusters.- Investigating Fungal Biosynthetic Pathways Using Heterologous Gene Expression - Aspergillus oryzae as a heterologous host.- Investigating Fungal Biosynthetic Pathways Using Heterologous Gene Expression: Aspergillus nidulans as a Heterologous Host.- Investigating Fungal Biosynthetic Pathways Using Heterologous Gene Expression: Fusarium sp. as a Heterologous Host.- Heterologous Expression of Fungal Biosynthetic Pathways in Aspergillus nidulans using episomal vectors.- Targeted Genetic Engineering via Agrobacterium-mediated transformation in Fusarium solani.- Investigating Fungal Biosynthetic Pathways Using Pichia pastoris as a Heterologous Host.- Evolutionary genome mining for the discovery and engineering of natural product biosynthesis.- Inducing Global Expression of Actinobacterial Biosynthetic Gene Clusters.- Engineering modular polyketide biosynthesis inStreptomyces using CRISPR/Cas: a practical guide.- CRISPR/Cas9-Based Methods for Inactivating Actinobacterial Biosynthetic Genes and Elucidating Function.- Understanding and Manipulating Assembly Line Biosynthesis by Heterologous Expression in Streptomyces.- Heterologous expression, purification, and characterization of type II polyketide acyl carrier proteins.- Cyanobacterial Genome Sequencing, Annotation and Bioinformatics.- Single crossover to inactivate target gene in cyanobacteria.- Double crossover approach to inactivate target gene in cyanobacteria.- Expression of Cyanobacterial Biosynthetic Gene Clusters in Escherichia coli.- Saccharomyces cerevisiae as a heterologous host for natural products.- Investigating Plant Biosynthetic Pathways Using Heterologous Gene Expression: Yeast as a Heterologous Host.- Rapid Combinatorial Co-Expression of Biosynthetic Genes by Transient Expression in the Plant Host Nicotiana benthamiana.- Optimized Tools and Methods for Methanotroph Genome Editing.- Microarray-based screening of putative Hsp90 inhibitors predicted, and isolated from microorganisms.- Isolation of Water-Soluble Metabolites from Marine Invertebrates and Microorganisms.- Natural Product Investigation in Lichens: Extraction and HPLC analysis of Secondary Compounds in Mycobiont Cultures.