Autophagy and Cancer

A balance between autophagy and other cell death modalities in cancer.- Isolation of Autophagy competent Lysosomes from Cancer cells by Differential Large-scale Multi-layered Density Gradient Centrifugations.- CRISPR-Cas9 Gene Editing to create Isoform specific LAMP-2A Knockout in Human Cancer Cells.- Modified LC3 dot quantification method.- A quantitative flow cytometry-based method for autophagy detection across the cell cycle.- A dual HiBiT-GFP-LC3 lentiviral reporter for autophagy flux assessment.- Measuring autophagic cargo flux with Keima-based probes.- Detection of nuclear biomarkers for chromosomal instability.- Analysis of autophagic vesicles in mitotic cells.- Approaches and protocols to analyze autophagy and its role in death of apoptosis-resistant senescent tumor cells.- Concepts and design of introducing synthetic microRNAs into mammalian cells.- Studying autophagy using a TMT-based quantitative proteomics approach.- Immunofluorescence-based measurement of autophagosome formation during mitophagy.- Induction and detection of mitophagy.- Analysis of pancreatic acinar protein solubility in autophagy-deficient mice.- Studying autophagy in vivo in the mammary gland and in xenograft samples.- A method to Identify Potential Prognostic Markers Across Distinct Tumor Types.- A method for Co-Expression Analysis.- Methodological approaches for assessing metabolomic changes in glioblastomas -MS.- Extraction of Metabolites from Cancer Cells.- Measuring glucose consumption and gluconeogenesis in 3D human tissue cultures with nanoliter input volumes.