The Integrated Stress Response
Springer-Verlag New York Inc.
978-1-0716-1974-2 (ISBN)
Authoritative and practical, The Integrated Stress Response: Methods and Protocols serves as an ideal guide to help accelerate research into the complex and fascinating biology of the ISR.
An Overview of Methods for Detecting eIF2α Phosphorylation and the Integrated Stress Response.- CRISPR-Based Screening for Stress Response Factors in Mammalian Cells.- Multiplexed Analysis of Human uORF Regulatory Functions during the ISR Using PoLib-Seq.- Measuring Bulk Translation Activity in Single Mammalian Cells during the Integrated Stress Response.- Quantitative Translation Proteomics Using mePROD.- Quantifying the Binding of Fluorescently Labelled Guanine Nucleotides and Initiator tRNA to Eukaryotic Translation Initiation Factor 2.- Mammalian In Vitro Translation Systems.- Measuring Repeat-Associated Non-AUG (RAN) Translation.- Analysis of Ribosome Profiling Data.- Analysis of Translational Control in the Integrated Stress Response by Polysome Profiling.- High-Resolution Ribosome Profiling for Determining Ribosome Functional States during Translation Elongation.- Fluorescence Intensity-Based eIF2B’s Guanine Nucleotide-Exchange Factor Activity Assay.- Collective Learnings of Studies of Stress Granule Assembly and Composition.- Detecting Stress Granules in Drosophila Neurons.- Monitoring and Quantification of the Dynamics of Stress Granule Components in Living Cells by Fluorescence Decay after Photoactivation.- Probing Protein Solubility Patterns with Proteomics for Insight into Network Dynamics.- Analyzing the Composition and Organization of Ribonucleoprotein Complexes by APEX-Seq.- Differential Analysis of the Nuclear and the Cytoplasmic RNA Interactomes in Living Cells.- Identification of RNA-RBP Interactions in Subcellular Compartments by CLIP-Seq.- Monitoring Virus-Induced Stress Granule Dynamics Using Long-Term Live-Cell Imaging.- Single-Molecule Imaging of mRNA Interactions with Stress Granules.- Image-Based Screening for Stress Granule Regulators.- APEX Proximity Labeling of Stress Granule Proteins.
Erscheinungsdatum | 09.03.2022 |
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Reihe/Serie | Methods in Molecular Biology ; 2428 |
Zusatzinfo | 17 Illustrations, black and white; XII, 404 p. 17 illus. With online files/update. |
Verlagsort | New York, NY |
Sprache | englisch |
Maße | 178 x 254 mm |
Themenwelt | Medizin / Pharmazie ► Medizinische Fachgebiete ► Arbeits- / Sozial- / Umweltmedizin |
Studium ► Querschnittsbereiche ► Klinische Umweltmedizin | |
Naturwissenschaften ► Biologie ► Genetik / Molekularbiologie | |
Naturwissenschaften ► Biologie ► Mikrobiologie / Immunologie | |
Naturwissenschaften ► Biologie ► Zellbiologie | |
Schlagworte | eIF2 kinases • eIF2α kinases • ISR • Processing bodies • Stress granules • Stress response genes • structural biology |
ISBN-10 | 1-0716-1974-8 / 1071619748 |
ISBN-13 | 978-1-0716-1974-2 / 9781071619742 |
Zustand | Neuware |
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