Periodontal Pathogens

Site-Directed and Random Mutagenesis in Porphyromonas gingivalis: Construction of Fimbriae-Related-Gene Mutant.- Genetic Manipulations of Oral Spirochete Treponema denticola.- Construction of a Gene-Deletion Mutant in Tannerella forsythia.- Construction of a Mutant in Prevotella melaninogenica Using the Conjugation Transfer Method with Escherichia coli.- Genetic Transformation of Fusobacterium nucleatum.- Genotyping of Porphyromonas gingivalis in Relationship to Virulence.- Transport and Polymerization of Porphyromonas gingivalis Type V Pili.- Purification of Native Mfa1 Fimbriae from Porphyromonas gingivalis.- Crystallization of Recombinant Fimbrial Proteins of Porphyromonas gingivalis.- Enzymatic Characteristics and Activities of Gingipains from Porphyromonas gingivalis.- Structural Characterization of the Type IX Secretion System in Porphyromonas gingivalis.- Methods for Functional Characterization of the Type IX Secretion System of Porphyromonas gingivalis.- Purification of Tannerella forsythia Surface (S-) Layer Proteins.- Separation of Glycosylated OmpA-Like Proteins from Porphyromonas gingivalis and Tannerella forsythia.- Intranasal Vaccine Study Using Porphyromonas gingivalis Membrane Vesicles: Isolation Method and Application to a Mouse Model.- Analysis of the Butyrate-Producing Pathway in Porphyromonas gingivalis.- Characterization of the Treponema denticola Virulence Factor Dentilisin.- Evaluation of the Virulence of Aggregatibacter actinomycetemcomitans through the Analysis of Leukotoxin.- Lipoprotein Extraction from Microbial Membrane and Lipoprotein/Lipopeptide Transfection into Mammalian Cells.- Analysis of the Interaction between HIV and Periodontopathic Bacteria that Reactivates HIV Replication in Latently Infected Cells.- Invasion of Gingival Epithelial Cells by Porphyromonas gingivalis.- Analysis ofInteraction between Porphyromonas gingivalis and Endothelial Cells In Vitro.- Analysis of Experimental Ligature-Induced Periodontitis Model in Mice.