Methods in Bone Biology

Culture of cells of the osteoblast lineage.- In vitro models for osteoclast recruitment.- Isolation and culture of osteoclasts and osteoclast resorption assays.- Bone organ cultures.- Methods for studying cell death in bone.- Models for mechanical loading of hone and hone cells in vivo and in vitro.- Bone histomorphometry.- The application of immunocytochemistry and in situ hybridization to cryostat sections of undecalcified bone.- Biochemical markers of bone turnover.- Radiographic measurement of bone turnover: microfocal radiography.- Bone mineral measurements by DXA in animals.- Animal models for the investigation of the action of factors on bone metabolism.