Molecular Dermatology

Isolation of Epidermal Keratinocytes from Human Skin: The Scratch-Wound Assay for Assessment of Epidermal Keratinocyte Migration.- Isolation of Different Dermal Fibroblast Populations from the Skin and the Hair Follicle.- Isolation of Epidermal and Hair Follicle Melanocytes.- Isolation and Culture of Human Skin Mast Cells.- Organotypic 3D Skin Models: Human Epidermal Equivalent Cultures from Primary Keratinocytes and Immortalized Keratinocyte Cell Lines.- Purification of Extracellular Microvesicles Secreted by Dermal Fibroblasts.- A Method to Investigate the Epidermal Permeability Barrier In Vitro.- Isolating Dermal Papilla Cells from Human Hair Follicles Using Microdissection and Enzyme Digestion.- Methods to Study Human Hair Follicle Growth Ex Vivo: Human Microdissected Hair Follicle and Human Full Thickness Skin Organ Culture.- Non-Surgical Induction of Alopecia Areata in C3H/HeJ Mice via Adoptive Transfer of Cultured Lymphoid Cells.- Collapse and Restoration of Hair Follicle ImmunePrivilege Ex Vivo: A Model for Alopecia Areata.- Experimentally-Induced Epithelial-Mesenchymal Transition of Human Hair Follicle Stem Cells as a Model of Scarring Alopecia Ex Vivo.- Generation of Hair Follicle Germs in Vitro Using Human Postnatal Skin Cells.- Method for Human Eccrine Sweat Gland Isolation from the Scalp by Means of the Micro-Punch Technique and Vital Dyes.- Identification of Long Non-Coding RNA by In Situ Hybridization Approaches.- Detection of MicroRNAs by In Situ Hybridization in Skin.- Chromatin Immunoprecipitation of Low Number of FACS-Purified Epidermal Cells.- 3D-FISH Analysis of the Spatial Genome Organization in Skin Cells In Situ.- Method to Study Skin Cancer: Two-Stage Chemically-Induced Carcinogenesis in Mouse Skin.- Investigation of Skin Wound Healing Using a Mouse Model.- Human Wound Healing Ex Vivo Model with Focus on Molecular Markers.- Method for Investigation of Photobiological Effects of Light on Human Skin Cells Mediated by Low Doses of Light.