Methods for Fragments Screening Using Surface Plasmon Resonance

Dr. Ramachandraiah Gosu is Associate Director, Discovery Biology & Head of Structural Biology Department at Jubilant Biosys Ltd, Bengaluru. His research interests are protein engineering, macromolecular X-ray crystallography and methods for biophysical screening.  Before joining Jubilant Biosys, he worked as a post-doctoral fellow at the University of Minnesota, Minneapolis in the Structural Genomics Project- Pathogenic Island Proteins from Staphylococcus aureus. He has obtained his Ph.D. in, Structural Biology from Molecular Biophysics Unit at the Indian Institute of Science, Bangalore, India.  He has co-authored several articles in peer-reviewed international journals. Dr. Sameer Mahmood. Z is Group Leader at the Jubilant Biosys Ltd, Bengaluru, India. He has earlier served as Associate Principal Scientist in the Department of invitro biology at GVK Bioscience Ltd, Hyderabad, India, and Principal Scientist I in the Department of Molecular and Cell Biology, Advinus Therapeutics Ltd, Pune. He also did postdoctoral training in the Department of Stem Cell and Molecular Biology at Joslin Diabetes Center (Harvard University), Boston, USA. His research interest is in the high throughput screening of proteins using robotics with specialization in protein-drug (Fragments and Small molecules) interactions. He has been accolade with several awards in Stem cell research, Jackson Laboratory (USA), Asia ARVO (Singapore), Research Achievement Award (USA), CEO award for Excellence in Project Management (India). He has published several research articles in peer-reviewed international journals and co-authored a book chapter.

Chapter 1_Introduction to SPR.- Chapter 2_ Principle of SPR (OneStep).- Chapter 3_Identification of Fragment library.- Chapter 4_Preparation of protein with Histidine Tag.- Chapter 5_Preparation of protein with Biotin Tag.- Chapter 6_Protein stability using Thermal Shift Assay (TSA) - pH.- Chapter 7_Protein stability using Thermal Shift Assay (TSA) – DMSO.- Chapter 8_Preparation of Sensor.- Chapter 9_ Optimization and validation of Amine coupling.- Chapter 10_ Optimization and validation of Biotin-strepavidin coupling.- Chapter 11_ Preparation of Fragment for Screening in SPR.- Chapter 12_ Screening of Fragment in SPR.- Chapter 13_ Analysis of Data.- Chapter 14_ Identification and arraying of HITS.- Chapter 15_ Confirmation of Hits – Kinetics (kon, koff, KD, LE).