Yarrowia lipolytica
Springer-Verlag New York Inc.
978-1-0716-1413-6 (ISBN)
Authoritative and cutting-edge, Yarrowia lipolytica: Methods and Protocols aims to provide experimentalists with a full account of the practical steps necessary for carrying out each protocol successfully.
Genome Engineering of Yarrowia lipolytica with the piggyBac Transposon System.- Genome Editing in Y. lipolytica Using TALENs.- EasyCloneYALI: Toolbox for CRISPR-mediated Integrations and Deletions in Yarrowia lipolytica.- Simultaneous gene excision and integration by dual-guide CRISPR-Cas9.- Gene excision by dual-guide CRISPR-Cas9.- CRISPR Interference and Activation to Modulate Transcription in Yarrowia lipolytica..- Implementing CRISPR-Cas12a for Efficient Genome-editing in Yarrowia lipolytica.- Guide RNA Design for Genome-wide CRISPR Screens in Yarrowia lipolytica.- Targeted Integration through Transformation of Hydroxyurea-Arrested Cells.- Strain Construction for Intracellular Metabolic Pathway Localization in Y. lipolytica.- Engineering Yarrowia lipolytica for production of fatty alcohols with YaliBrick Vectors.- Methods to Activate and Elucidate ComplexEndogenous Sugar Metabolism in Yarrowia lipolytica.- Pathway Engineering For Beta-Carotene and Carotenoid Biosynthesis in Y. lipolytica.- Droplet-based Microfluidic High-throughput Screening of Enzyme Mutant Libraries Secreted by Yarrowia lipolytica.- Yarrowia lipolytica Bioprocess Development – From Flask to Bioreactor.- Western Blotting of Membrane-Bound Proteins in Yarrowia lipolytica.
Erscheinungsdatum | 16.04.2021 |
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Reihe/Serie | Methods in Molecular Biology ; 2307 |
Zusatzinfo | 44 Illustrations, color; 21 Illustrations, black and white; X, 250 p. 65 illus., 44 illus. in color. |
Verlagsort | New York, NY |
Sprache | englisch |
Maße | 178 x 254 mm |
Themenwelt | Medizin / Pharmazie ► Medizinische Fachgebiete |
Studium ► 2. Studienabschnitt (Klinik) ► Humangenetik | |
Schlagworte | CRISPR-Cas9 • Genetic Engineering • Lipid analysis • systems biology • Talen |
ISBN-10 | 1-0716-1413-4 / 1071614134 |
ISBN-13 | 978-1-0716-1413-6 / 9781071614136 |
Zustand | Neuware |
Informationen gemäß Produktsicherheitsverordnung (GPSR) | |
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