Antibody-Drug Conjugates

An Overview of the Current ADC Discovery Landscape.- Pushing the Envelope: Advancement of ADCs Outside of Oncology.- Conjugations to Endogenous Cysteine Residues.- Site-Specific Conjugation to Cys-Engineered THIOMAB™ Antibodies.- Transglutaminase-Mediated Conjugations.- Click Chemistry Conjugations.- Utilizing Solid-Phase to Enable High-Throughput, Site-Specific Conjugation and Dual Labeled Antibody and Fab Conjugates.- Bridged Cysteine Conjugations.- Antibody Conjugations via Glycosyl Remodeling.- ADC Analysis by Hydrophobic Interaction Chromatography.- Two-Dimensional Liquid Chromatography Coupled to High Resolution Mass Spectrometry for the Analysis of ADCs.- Drug Loading and Distribution of ADCs after Reduction or IdeS Digestion and Reduction.- Analysis of ADCs by Native Mass Spectrometry.- High Resolution Characterization of ADCs by Orbitrap LCMS.- Conjugation Site Analysis by MS/MS Protein Sequencing.- Conjugation Site Analysis of Lysine-Conjugated ADCs.- Characterization of ADCs by Capillary Electrophoresis.- Characterization of the Primary Structure of Cysteine-Linked Antibody-Drug Conjugates using Capillary Electrophoresis with Mass Spectrometry.- Purification of ADCs by Hydrophobic Interaction Chromatography.- Detection and Removal of Small-Molecule and Endotoxin Contaminants in ADC Preparations.- Physical Stability Studies of Antibody Drug Conjugates (ADCs) Under Stressed Conditions.- Biophysical Methods for Characterization of Antibody Drug Conjugates.- Determination of ADC Cytotoxicity in Immortalized Human Cell Lines.- LC/MS Methods for Studying Lysosomal ADC Catabolism.- Assessing ADC Plasma Stability by LC/MS Methods.- Determination of ADC Concentration by Ligand-Binding Assays.