Regulatory B Cells

 Purification and characterization of murine MZ and T2-MZP cells.- Purification and immune-phenotyping of B-1 cells from body cavities of mice.- Purification and immunophenotypic characterization of murine plasma cells.- Purification of murine and human IL-10 producing B cells from different anatomical compartments.- Purification and immunophenotypic characterization of human CD19+CD24hiCD38hi and CD19+CD24hiCD27+ B cells.- Detection of IL-10 in murine B cells: in vitro and in vivo techniques.- Detection and quantification of transforming growth factor-β1 produced by murine B cells: pros and cons of different techniques.- IL-35 detection in B cells at the mRNA and protein level.- Characterization and activation of Fas Ligand producing mouse B cells and their killer exosomes.- Characterization and activity of TIM-1 and IL-10-reporter expressing regulatory B cells.- New method for the expansion of highly purified human regulatory granzyme B-expressing B cells.- Characterization of the cell surface phenotype and regulatory activity of B cell IgD low (BDL) regulatory B cells.- Use of Toll-like receptor (TLR) ligation to characterize human regulatory B cells subsets.- Use of co-cultures for the study of cellular interactions influencing B cell regulatory functions.- Use of inhibitory compounds to dissect the molecular pathways involved in regulatory B cell differentiation.- Methods to study the transcriptome of regulatory B cells.- Purification of murine IL-10+ B cells for analyses of biological functions and transcriptomics.-  il-10 gene locus DNA methylation in regulatory B cells.- B cell commitment to IL-10 production: the Vert-X Il10egfp mouse.- Regulatory B cells in experimental mouse models of arthritis.- Regulatory and IgE+ B cells in allergic asthma.- Regulatory B cells in type 1 diabetes.-  Analysis of regulatory B cells in experimental autoimmune uveitis.- Purification and immunophenotypic characterization of human CD24hiCD38hi and CD24hiCD27+ regulatory B cells in transplantation.