G Protein-Coupled Receptor Screening Assays

Isolation of Lipid Rafts by the Detergent and Non-Detergent Based Methods for Localization of GPCRs with Immunoblotting and Laser Scanning Confocal Microscopy.- Detection of GPCR mRNA Expression in Primary Cells via qPCR, Microarrays, and RNA-Sequencing.- Construction of Recombinant Cell Lines for GPCR Expression.- Recombinant Expression and Purification of Cannabinoid Receptor CB2, a G Protein-Coupled Receptor.- Screening for Serotonin Receptor 4 Agonists Using GPCR-Based Sensor in Yeast.- Immobilization of Olfactory Receptors Carried by Nanosomes onto a Gold Sensor Surface.- Screening Methods for Cell-Free Synthesized GPCR/Nanoparticle Samples.- Fluorescence Anisotropy-Based Assay for Characterization of Ligand Binding Dynamics to GPCRs: The Case of Cy3B-Labelled Ligands Binding to MC4 Receptors in Budded Baculoviruses.- Bioluminescence in G Protein-Coupled Receptors Drug Screening Using Nanoluciferase and Halo-Tag Technology.- Nanoluciferase-Based Complementation Assay to Detect GPCR-G Protein Interaction.- Imaging of Genetically-Encoded FRET-Based Biosensors to Detect GPCR Activity.- cAMP Biosensor Assay Using BacMam Expression System: Studying the Downstream Signaling of LH/hCG Receptor Activation.- FLIPR Calcium Mobilization Assays in GPCR Drug Discovery.- Live Cell Imaging and Optogenetics-Based Assays for GPCR Activity.- Split-Tobacco Etch Virus (Split-TEV) Method in G Protein-Coupled Receptor Interacting Proteins.- NanoLuc-Based Methods to Measure β-Arrestin2 Recruitment to G Protein-Coupled Receptors.- Luciferase Complementation Approaches to Measure GPCR Signaling Kinetics and Bias.- Gradient Tracking by Yeast GPCRs in a Microfluidics Chamber.- Monitoring Intracellular Calcium in Response to GPCR Activation: Comparison Between Microtiter Plates and Microfluidic Assays.- Homology Modeling Using GPCRM Web Service.