Cell Cycle Checkpoints
Springer-Verlag New York Inc.
978-1-0716-1216-3 (ISBN)
Cutting-edge and thorough, Cell Cycle Checkpoints: Methods and Protocols is a valuable resource for researcher interested in learning more about this developing field.
Determination of CHK1 Cellular Localization by Immunofluorescence Microscopy.- Detection of Post-Translationally Modified p53 by Western Blotting.- Global Analyses to Identify Direct Transcriptional Targets of p53.- Analysis of Replication Dynamics Using the Single-Molecule DNA Fiber Spreading Assay.- Measuring Translation Efficiency by RNA Immunoprecipitation of Translation Initiation Factors.- DNA Affinity Purification: A Pulldown Assay for Identifying and Analyzing Proteins Binding to Nucleic Acids.- A Novel Strategy to Track Lysine-48 Ubiquitination by Fluorescence Resonance Energy Transfer.- The DNA Damage Response in Xenopus laevis Cell-Free Extracts.- Mammalian Cell Fusion Assays for the Study of Cell Cycle Progression by Functional Complementation.- Knockdown of Target Genes by siRNA In Vitro.- Assaying Cell Cycle Status Using Flow Cytometry.- Using TUNEL Assay to Quantitate p53-Induced Apoptosis in Mouse Tissues.- Generation and Analysis of dsDNA Breaks for Checkpoint and Repair Studies in Fission Yeast.- Calreticulin Exposure in Mitotic Catastrophe.- Quantification of eIF2α Phosphorylation Associated with Mitotic Catastrophe by Immunofluorescence Microscopy.- Clonogenic Assays to Detect Cell Fate in Mitotic Catastrophe.
Erscheinungsdatum | 26.03.2021 |
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Reihe/Serie | Methods in Molecular Biology ; 2267 |
Zusatzinfo | 21 Illustrations, color; 10 Illustrations, black and white; XI, 242 p. 31 illus., 21 illus. in color. |
Verlagsort | New York, NY |
Sprache | englisch |
Maße | 178 x 254 mm |
Themenwelt | Studium ► Querschnittsbereiche ► Infektiologie / Immunologie |
Naturwissenschaften ► Biologie ► Mikrobiologie / Immunologie | |
Naturwissenschaften ► Biologie ► Zellbiologie | |
ISBN-10 | 1-0716-1216-6 / 1071612166 |
ISBN-13 | 978-1-0716-1216-3 / 9781071612163 |
Zustand | Neuware |
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