Methicillin-Resistant Staphylococcus Aureus (MRSA) Protocols

Cutting-Edge Technologies and Advancements

Yinduo Ji (Herausgeber)

Buch | Softcover

268 Seiten

2020 | 3rd ed. 2020
Humana Press Inc. (Verlag)
978-1-4939-9851-7 (ISBN)

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This third edition volume expands on the previous editions with an update on the latest techniques used for the detection, genotyping, and investigating pathogenesis of Staphylococcus aureus in vitro and in vivo. The methods covered in this book mostly focus on routine clinical diagnosis, surveillance, research, and practice for treatment of patients infected by multi-drug resistant S. aureus. The book also covers the epidemiology of MRSA, molecular typing approaches, clinical treatment of MRSA infections, and animal models of drug discovery. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Informative and cutting-edge, Methicillin-Resistant Staphylococcus Aureus (MRSA) Protocols: Cutting-Edge Technologies and Advancements, Third Edition is a valuable resource for researchers looking to set up new methods to study S. aureus, and will also be very useful for technicians and scientists working on other bacterial pathogens.

Clinical, Epidemiologic, and Laboratory Aspects of Methicillin-Resistant Staphylococcus aureus Infections.- Rapid Methods for Detection of MRSA in Clinical Specimens.- Immunofluorescence Microscopy for the Detection of Surface Antigens in Methicillin Resistant Staphylococcus aureus.- Staphylococcal Cassette Chromosome mec (SCCmec) Analysis of MRSA.- Pulsed-Field Gel Electrophoresis Typing of Staphylococcus aureus Strains.- Spa Genotyping of Staphylococcus aureus Isolates.- Multilocus Sequence Typing of Staphylococcus aureus.- Genetic Manipulations of Staphylococcal Chromosomal DNA.- Genetic Manipulation of MRSA using CRISPR/Cas9 Technology.- RNA-seq Identification of Target Genes Mediated by Regulators of Staphylococcus aureus.- Application of Two-Dimensional Difference Gel Electrophoresis in Identification of Factors Responsible for Virulence of Staphylococcus aureus.- Identification of Virulence Determinants during Host-Pathogen Interaction using Tn-Seq Technology.- Metabolomic Profiling of Staphylococcus aureus.- Determining Impact of Growth Phases on Capacity of Staphylococcus aureus to Adhere to and Invade Host Cells.- Preclinical Models and Methodologies for Monitoring Staphylococcus aureus Infections using Noninvasive Optical Imaging.- Methicillin-Resistant Staphylococcus aureus Infection and Treatment Options.- Animal Models for Drug Development for MRSA.

Erscheinungsdatum	18.09.2020
Reihe/Serie	Methods in Molecular Biology ; 2069
Zusatzinfo	27 Illustrations, color; 8 Illustrations, black and white; XI, 268 p. 35 illus., 27 illus. in color.
Verlagsort	Totowa, NJ
Sprache	englisch
Maße	178 x 254 mm
Themenwelt	Medizin / Pharmazie ► Medizinische Fachgebiete
	Studium ► Querschnittsbereiche ► Infektiologie / Immunologie
	Naturwissenschaften ► Biologie ► Mikrobiologie / Immunologie
Schlagworte	Animal model • antibiotic resistance • MSSA • Surface antigens • Tn-Seq
ISBN-10	1-4939-9851-X / 149399851X
ISBN-13	978-1-4939-9851-7 / 9781493998517
Zustand	Neuware