Cryptosporidium
Humana Press Inc. (Verlag)
978-1-4939-9750-3 (ISBN)
Authoritative and practical, Cryptosporidium: Methods and Protocols serves as an idea guide to the inherent challenges of working with cryptosporidial parasites to provide a foundation for new investigators to build upon.
The chapter “Accessing Cryptosporidium Omic and Isolate Data via CryptoDB.org” is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.
Cryptosporidium Diagnostic Assays: Microscopy.- Cryptosporidium Diagnostic Assays: Molecular Detection.- Detection of Cryptosporidium Recovered From Large-Volume Water Samples Using Dead-End Ultrafiltration.- Cryptosporidium Oocyst Purification Using Discontinuous Gradient Centrifugation.- Measuring Cryptosporidium Serologic Responses by Multiplex Bead Assay.- Production and Purification of Functional Cryptosporidium Glycoproteins by Heterologous Expression in Toxoplasma gondii.- Cryptosporidium Genotyping for Epidemiology Tracking.- Use of Markers to Determine Cryptosporidium Genotypes for Epidemiology Tracking and Detection.- Preparation of Cryptosporidium DNA for Whole Genome Sequencing.- Accessing Cryptosporidium Omic and Isolate Data Via CryptoDB.org.- A Novel Method to Silence Genes in Cryptosporidium.- Use of miRNAs to Study Host Cell-Parasite Interactions.- Genetic Manipulation of Cryptosporidium parvum with CRISPR/Cas9.- Mouse Models for Use in Cryptosporidium Infection Studies and Quantification of Parasite Burden Using Flow Cytometry, qPCR, and Histopathology.- Calf Clinical Model of Cryptosporidiosis for Efficacy Evaluation of Therapeutics.- Cell Culture Infectivity to Assess Chlorine Disinfection of Cryptosporidium Oocysts in Water.- High-Content Screening for Cryptosporidium Drug Discovery.- High-Throughput Screening of Drugs Against the Growth of Cryptosporidium parvum In Vitro by qRT-PCR.- In Vitro Culture of Cryptosporidium parvum Using Hollow Fiber Bioreactor: Applications for Simultaneous Pharmacokinetic and Pharmacodynamic Evaluation of Test Compounds.- In Vitro Culture of Cryptosporidium parvum Using Stem Cell-Derived Intestinal Epithelial Monolayers.- Two and Three Dimensional Bioengineered Human Intestinal Tissue Models for Cryptosporidium.
Erscheinungsdatum | 29.08.2020 |
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Reihe/Serie | Methods in Molecular Biology ; 2052 |
Zusatzinfo | 71 Illustrations, color; 22 Illustrations, black and white; XI, 407 p. 93 illus., 71 illus. in color. |
Verlagsort | Totowa, NJ |
Sprache | englisch |
Maße | 178 x 254 mm |
Themenwelt | Medizin / Pharmazie ► Medizinische Fachgebiete ► Mikrobiologie / Infektologie / Reisemedizin |
Studium ► Querschnittsbereiche ► Infektiologie / Immunologie | |
Naturwissenschaften ► Biologie ► Mikrobiologie / Immunologie | |
Schlagworte | Anti-cryptosporidial drugs • CRISPR/Cas9 • genotyping • Host-Parasite Interactions • Intestinal tract infection • Oocysts • protozoan |
ISBN-10 | 1-4939-9750-5 / 1493997505 |
ISBN-13 | 978-1-4939-9750-3 / 9781493997503 |
Zustand | Neuware |
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