Human Cytomegaloviruses
Springer-Verlag New York Inc.
978-1-0716-1110-4 (ISBN)
Authoritative and up-to-date, Human Cytomegaloviruses: Methods and Protocols, Second Edition serves as an ideal scientific reference for basic and clinical scientists and medical personnel on the modern understanding of the pathobiology of the virus, and the approaches, techniques, and models to study human cytomegalovirus infection and disease.
Overview of Human Cytomegalovirus Pathogenesis.- Distinct Properties of Human Cytomegalovirus Strains and the Appropriate Choice of Strains for Particular Studies.- Use of Diploid Human Fibroblasts as a Model System to Culture, Grow, and Study Human Cytomegalovirus Infection.- The Use of Primary Human Cells to Analyze Human Cytomegalovirus Biology.- Human Hematopoietic Long-Term Culture (hLTC) for Human Cytomegalovirus Latency and Reactivation.- Collection and Isolation of CD14+ Primary Human Monocytes via Dual Density Centrifugation as a Model System to Study Human Cytomegalovirus Infection and Pathogenesis.- Stable and Inducible Gene Knockdown in Primary Human Fibroblasts: A Versatile Tool to Study the Role of Human Cytomegalovirus Host Cell Factors.- Construction of Human Cytomegalovirus Mutants with Markerless BAC Mutagenesis.- Methods for Studying the Function of Cytomegalovirus GPCRs.- Analysis of Cytomegalovirus Glycoprotein and Cellular Receptor Interactions.- Antibody-Independent Quantification of Cytomegalovirus Virion Protein Incorporation Using HiBiT.- Use of a Phosphoproteomic Screen to Profile Early Changes during HCMV Infection of Human Monocytes.- A Generally Applicable CRISPR/Cas9 Screening Technique to Identify Host Genes Required for Virus Infection as Applied to Human Cytomegalovirus (HCMV) Infection of Epithelial Cells.- Quantitative Electron Microscopy to Study HCMV Morphogenesis.- Detection of Cytomegalovirus Interleukin 10 (cmvIL-10) by Enzyme-Linked Immunosorbent Assay (ELISA).- Techniques for Characterizing Cytomegalovirus-Encoded miRNAs.- Development of a huBLT Mouse Model to Study HCMV Latency, Reactivation, and Immune Response.- Rodent Models of Congenital Cytomegalovirus Infection.- Recent Approaches and Strategies in the Generation of Anti-Human Cytomegalovirus Vaccines.
Erscheinungsdatum | 24.02.2021 |
---|---|
Reihe/Serie | Methods in Molecular Biology ; 2244 |
Zusatzinfo | 26 Illustrations, color; 15 Illustrations, black and white; XI, 468 p. 41 illus., 26 illus. in color. |
Verlagsort | New York, NY |
Sprache | englisch |
Maße | 178 x 254 mm |
Themenwelt | Medizin / Pharmazie ► Medizinische Fachgebiete ► Mikrobiologie / Infektologie / Reisemedizin |
Studium ► 1. Studienabschnitt (Vorklinik) ► Physiologie | |
Naturwissenschaften ► Biologie ► Mikrobiologie / Immunologie | |
ISBN-10 | 1-0716-1110-0 / 1071611100 |
ISBN-13 | 978-1-0716-1110-4 / 9781071611104 |
Zustand | Neuware |
Haben Sie eine Frage zum Produkt? |
aus dem Bereich