Bone Research Protocols

Part I. Culture of Osteoblasts and Osteocytes Human Osteoblast Culture James A. Gallagher Osteoblast Isolation from Murine Calvariae and Long Bones Astrid Bakker and Jenneke Klein-Nulend Mineralizing Fibroblast-Colony-Forming Assays Andrew Scutt, Lynsey Reading, Nanette Scutt, and Karen Still Osteocyte Isolation and Culture Peter J. Nijweide, Arie van der Plas, Marcel J. Alblas, and Jenneke Klein-Nulend Part II. Culture of Osteoclasts. Isolated Osteoclast Cultures Astrid Hoebertz and Timothy R. Arnett Primary Isolation and Culture of Chicken Osteoclasts Patricia Collin-Osdoby, Fred Anderson, and Philip Osdoby Isolation and Purification of Rabbit Osteoclasts Fraser P. Coxon, Julie C. Frith, Helena L. Benford, and Michael J. Rogers Generating Human Osteoclasts from Peripheral Blood Afsie Sabokbar and Nicholas A. Athanasou Generating Human Osteoclasts from Bone Marrow and Peripheral Blood Adrienne M. Flanagan and Helen M. Massey Generating Murine Osteoclasts from Bone Marrow Naoyuki Takahashi, Nobuyuki Udagawa, Sakae Tanaka, and Tatsuo Suda Osteoclast Formation in the Mouse Coculture Assay Robert J. van 't Hof RANKL-Mediated Osteoclast Formation from Murine RAW 264.7 Cells Patricia Collin-Osdoby, Xuefeng Yu, Hong Zheng, and Philip Osdoby Part III. Bone Organ Culture Analysis of Osteoclast Function in Mouse Calvarial Cultures Michael J. Marshall and Marit N. Rowlands Assessing Bone Formation Using Mouse Calvarial Organ Culture I. Ross Garrett Part IV. Histological and Ultrastructural Techniques In Situ Hybridization and In Situ Reverse Transcription Polymerase Chain Reaction in Human Bone Sections Andrew P. Mee and Judith A. Hoyland Techniques for the Study of Apoptosis in Bone Brendon S. Noble and Hazel Y. Stevens Protein Localization in Wax-Embedded and Frozen Sections of Bone Using Immunohistochemistry Sharyn Bord Detection of Noncollagenous Bone Proteins in Methylmethacrylate-Embedded Human Bone Sections Johannes P. T. M. van Leeuwen and Pieter Derkx Fluorescence Imaging of Bone-Resorbing Osteoclasts by Confocal Microscopy Stephen A. Nesbitt and Michael A. Horton Bone Histomorphometry Shobna Vedi and Juliet Compston Transmission Electron Microscopy of Bone Vincent Everts, Anneke Niehof, and Wouter Beertsen Scanning Electron Microscopy of Bone Deborah Marshall, Miep H. Helfrich, and Richard M. Aspden Part V. Imaging Techniques Bone Measurement by Peripheral Quantitative Computed Tomography in Rodents Jurg A. Gasser Part VI. In Vivo Techniques. Studies of Local Bone Remodeling: The Calvarial Injection Assay Robert J. van 't Hof, Claire E. Clarkin, and Kenneth J. Armour Inflammation-Induced Osteoporosis: The IMO Model Kenneth J. Armour and Katharine E. Armour Ovariectomy and Estrogen Replacement in Rodents Jade W. M. Chow Part VII. Mechanical Loading Techniques Mechanical Testing of Bone Ex Vivo Richard M. Aspden Bone Cell Responses to Fluid Flow Hazel Y. Stevens and John A. Frangos Methods for Analyzing Bone Cell Responses to Mechanical Loading Using In Vitro Monolayer and Organ Culture Models Andrew A. Pitsillides, Victoria Das-Gupta, Dominic Simon, and Simon C. F. Rawlinson Part VIII. Molecular Analysis Extraction of Nucleic Acids from Bone Tracy L. Stewart and Val Mann Analysis of Gene Expression in Bone by Quantitative RT-PCR Deborah Ireland Index