DNA Cloning and Assembly

An Integrated Computer-Aided Design and Manufacturing Workflow for Synthetic Biology.- Combinatorial-Hierarchical DNA Library Design Using the TeselaGen DESIGN Module with j5.- The AssemblX Toolkit for Reliable and User-friendly Multi-gene Assemblies.- High-Throughput PCR for DNA Part Generation.- High-Throughput DNA Assembly Using Yeast Homologous Recombination.- Highly-Multiplexed, Semi-Automated Nextera Next Generation Sequencing (NGS) Library Preparation.- Generation of MoClo Standard Parts Using Golden Gate Cloning.- Assembly of Multigene Constructs using the Modular Cloning System MoClo.- Hierarchical Modular DNA Assembly using MetClo.- PaperClip DNA Assembly: Reduce, Reuse, Recycle.- Phytobricks: Manual and Automated Assembly of Constructs for Engineering Plants.- Mobius Assembly.- Design and Implementation of Multi-Protein Expression Constructs and Combinatorial Libraries using Start-Stop Assembly.- BASIC – A Simple and Accurate Modular DNA Assembly Method.- Joint Universal Modular Plasmids: A Flexible Platform for Golden Gate Assembly in Any Microbial Host.- A Step-by-Step Protocol for COMPASS, a Synthetic Biology Tool for Combinatorial Gene Assembly.- SCRaMbLE-in: A Fast and Efficient Method to Diversify and Improve the Yields of Heterologous Pathways in Synthetic Yeast.