Animal Cell Biotechnology

Near-Physiological Cell-Cycle Synchronization with Countercurrent Centrifugal Elutriation.- Turning Cells into Reagents: The Preparation of Assay Ready Cells for Routine Use in Bioassays.- Screening of Media Supplements for High-Performance Perfusion Cultures by Design of Experiment.- ambr® 15 Microbioreactors for CHO Cell Cultivation.- Using a Parallel Micro-Cultivation System (Micro-Matrix) as a Process Development Tool for Cell Culture Applications.- HEK293 Cell-Based Bioprocess Development at Bench Scale by Means of On-Line Monitoring in Shake Flasks (RAMOS and SFR).- Orbitally Shaken Single-Use Bioreactor for Animal Cell Cultivation: Fed-Batch and Perfusion Mode.- Development of Mammalian Cell Perfusion Cultures at Lab Scale: From Orbitally Shaken Tubes to Benchtop Bioreactors.- Perfusion Control for High Cell Density Cultivation and Viral Vaccine Production.- How to Produce mAbs in a Cube-Shaped Stirred Single-Use Bioreactor at 200 L Scale.- Generic Workflow for the Setup of Mechanistic Process Models.- Estimation of Process Model Parameters.- Efficient Optimization of Process Strategies with Model-Assisted Design of Experiments.- Design, Optimization, and Adaptive Control of Cell Culture Seed Trains.- High-Throughput Quantification and Glycosylation Analysis of Antibodies using Bead-Based Assays.- Surface Plasmon Resonance-Based Method for Rapid Product Sialylation Assessment in Cell Culture.- Analysis of Product Quality of Complex Polymeric IgM Produced by CHO Cells.- Raman Trapping Microscopy for Non-Invasive Analysis of Biological Samples.- An Optical Biosensor for Continuous Glucose Monitoring in Animal Cell Cultures.- Turbidimetry and Dielectric Spectroscopy as Process Analytical Technologies for Mammalian and Insect Cell Cultures.- Continuous Chromatography Purification of Virus-Based Biopharmaceuticals: A Shortcut Design Method.- Single Step Purification of Glycogen Synthase Kinase Isoforms from Small Scale Transient Expression in HEK293 Cells with a Calcium-Dependent Fragment Complementation System.