Humason's Animal Tissue Techniques

Janice Presnell is a histology technician with over 20 years of experience in histotechnology. Martin Schreibman is distinguished professor in the Brooklyn College department of biology at the City University of New York where he has taught animal histology and microtechnique for more than 30 years.

Part 1 Historical perspectives: overview of the development of microtechnology. Part 2 Laboratory safety: practical aspects of safety; special provisions for the handicapped. Part 3 Basic procedures: fixation; dehydration - preparation for embedding; clearing; infiltrating; embedding - paraffin method; microtomes and microtome knives; paraffin sectioning and mounting; the microscope; stains and staining action; mounting and staining procedures; haematoxylin staining. Part 4 Specific staining methods: staining connective tissue and muscle; silver impregnating reticulum; silver impregnating and staining neurological elements; periodic acid-Schiff (PAS) and Feulgen techniques and related reactions; staining haematologic elements and related tissues; staining pigments and minerals; staining proteins and nucleic acids; staining lipids and carbohydrates; staining cellular elements; staining golgi apparatus, mitochondria, and living cells; staining microorganisms. Part 5 Histochemistry and miscellaneous special procedures: histochemistry; immunohistochemical methods; microwave histology; special procedures I; special procedures II; special procedures III; special procedures. Part 6 Solution preparation and general laboratory aids: solution preparation; general laboratory aids. (Part contents)