Human Spermatozoa in Assisted Reproduction, Second Edition

Basic Concepts of Spermatology and Fertilization: Spermatogenesis and intratesticular sperm maturation; Anatomy of the mature spermatozoon; Physiological changes of sperm during transport along the female genital tract; The fertilization process in the human. Male Factor Diagnosis: The concept of male factor in assisted reproduction; Basic semen analysis; Evaluation of sperm motility; Evaluation of sperm morphology by light microscopy; by computer analysis; Efficiency of predictive value of different methods of morphological evaluation; Male genital tract infections; Antisperm antibodies; Sperm enzymes for diagnostic purposes; Determination of membrane integrity and viability; Assessment of nuclear maturity; Cervical mucus penetration; Human sperm acrosome reaction; Zona-free hamster-egg sperm penetration assay; Sperm-zona pellucid binding and penetration assays; Reactive oxygen species in the context of diagnostic andrology; Sperm separation techniques and X/Y sperm separation; Prediction of sperm fertilizing potential; Sperm membrane moieties; Chromosomes of human spermatocytes, sperm, and the male pronucleus. Male Factor Treatment: In vitro fertilization; Gamete intrafallopian transfer; Artificial insemination using doctor's sperm; using husband's sperm; Assisted fertilization; Epididymal microaspiration and in vitro fertilization; Systemic therapy of sperm defects; Sperm collection for assisted reproduction in ejaculatory dysfunctions; Therapeutic prospects in the treatment of male factor. Laboratory Techniques. Index.