Electroporation Protocols
Humana Press Inc. (Verlag)
978-1-4939-9739-8 (ISBN)
Authoritative and easily accessible, Electroporation Protocols: Microorganism, Mammalian System, and Nanodevice, Third Edition aims to be an invaluable resource for investigators both in and outside of this field.
Cell Size-specific Transfection by Micropillar Array Electroporation.- Soft Electroporation through 3D Hollow Nanoelectrodes.- Micromachining of Polymeric Microfluidic Micro-/Nano-Electroporation Device.- 3D Nanochannel Array for High-Throughput Cell Manipulation and Electroporation.- Low-Voltage Flow-Through Electroporation Membrane and Method.- Nanofountain Probe Electroporation for Monoclonal Cell Line Generation.- 3D Nanochannel Electroporation for Macromolecular Nucleotides Delivery.- Nanoelectroporation and Collection of Genetically Modified Exosomes in Primary Cultures of Dendritic Cells.- Electroporation of CRISPR-Cas9 into Malignant B Cells for Loss-of-function Studies of Target Gene via Knockout.- Microfluidic Device for Localized Electroporation.- Transdermal Delivery of Nucleic Acid Mediated by Punching and Electroporation.- Targeted in vivo Electroporation Using Nanoengineered Microelectrodes.- Zygote Electroporation for CRISPR/Cas9 Delivery to Generate Genetically Modified Mice.- Genome Editing in Staphylococcus aureus by Conditional Recombineering and CRISPR/Cas9-mediated Counterselection.- Tissue Nano-Transfection of Surgically Exposed Stroma.- Transformation of a Model Microalga, Chlamydomonas reinhardtii without Cell-wall Removal.- Electroporation Transformation Protocol for Phaeodactylum tricornutum.- Efficient Transformation of the Diatoms Phaeodactylum tricornutum by Multi-pulse Electroporation.- Rapid and Effective Electroporation Protocol for Nannochloropsis oceanica.- Electroporation Procedures for Genetic Modification of Green Algae Chlorella spp..- Electrotransformation of Saccharomyces cerevisiae.
Erscheinungsdatum | 13.09.2019 |
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Reihe/Serie | Methods in Molecular Biology ; 2050 |
Zusatzinfo | 31 Illustrations, color; 7 Illustrations, black and white; XI, 196 p. 38 illus., 31 illus. in color. |
Verlagsort | Totowa, NJ |
Sprache | englisch |
Maße | 178 x 254 mm |
Themenwelt | Medizin / Pharmazie ► Medizinische Fachgebiete ► Biomedizin |
Studium ► 2. Studienabschnitt (Klinik) ► Humangenetik | |
Schlagworte | Array Electroporation • CRISPR/Cas9 • dose control • mouse zygotes • Neuronal Network • single cell transfection |
ISBN-10 | 1-4939-9739-4 / 1493997394 |
ISBN-13 | 978-1-4939-9739-8 / 9781493997398 |
Zustand | Neuware |
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