Vaccinia Virus

Working Safely with Vaccinia Virus: Laboratory Technique and Review of Published Cases of Accidental Laboratory Infections with Poxviruses.- Bioinformatics for Analysis of Poxvirus Genomes.- Simple, Rapid Preparation of Poxvirus DNA for PCR Cloning and Analysis.- Construction and isolation of recombinant vaccinia virus expressing fluorescent proteins.- Generation of Vaccinia Virus Gene Deletion Mutants Using Complementing Cell Lines.- Vaccinia genome editing using CRISPR.-RNAi-mediated depletion of poxvirus proteins.- Assessing the Structure and Function of Vaccinia Virus Gene Products by Transient Complementation.- Preliminary Screening and In vitro confirmation of orthopoxvirus antivirals.- Vaccinia virus transcriptome analysis by RNA sequencing.- Ribosome profiling of vaccinia virus-infected cells.- Quantitative PCR-based Assessment of Vaccinia Virus RNA and DNA in Infected Cells.- Click Chemistry Based Labelling of Poxvirus Genomes.- Visualizing poxvirus replication and recombination using live cell imaging.- High-Content Analyses of Vaccinia Plaque Formation.- Super-Resolution Microscopy of Vaccinia Virus Particles.- Bioluminescence imaging as a tool for poxvirus biology.- Growth and Purification of Vaccinia Virus Stocks for Intravital Imaging.- Intravital Imaging of Vaccinia Virus-Infected Mice.