Allergy

Understanding of the Molecular Mechanisms of Allergy.- T-Cell: Primary Culture from Peripheral Blood.- Measurement of Allergen-Specific Inhibitory Antibody Activity.- Immunoelectrophoresis for the Characterization of Allergen Extracts.- Standardization of Allergen Extracts.- Quantifying Human Innate Cytokine and Chemokine Responses Ex Vivo via Pattern Recognition Receptor Stimulation.- Improved Methods for Quantifying Human Chemokine and Cytokine Biomarker Responses: Ultrasensitive ELISA and Meso Scale Electrochemiluminescence Assays.- Development of Mould Antigen-Specific Enzyme-Linked Immunosorbent Assays (ELISA) to Quantify Airborne Antigen Exposure.- Monocyte-Derived Dendritic Cells as Antigen Presenting Cells in T-Cell Proliferation and Cytokine Production.- The Use of Dual Cell Tracker Dye Staining for the Identification and Characterization of Peanut Specific T-Cell Subsets.- The Use of pMHCII Tetramer Technology for the Identification and Characterization of CD4+ T Cells.- Isolation and Characterization of Lymphocytes from Human Mucosal Biopsies.- Development of a Dipstick Assay for Soy Allergens: Inexpensive Detection to Control Allergen Exposure.- Novel Method for the Purification of House Dust Mite Allergen Der p 1 and Its Use in Structure-Based Chemical Design of Novel Inhibitors.- Measurement of Specific IgG Anti-Fel d 1 Antibodies.- Measurement of Specific IgG4 Anti-Mouse Urine Antibodies.- Preparation of Samples for a Mass Spectrometry Based Method to Identify Allergenic Proteins.