Pathogenic Yersinia

An intradermal model for Yersinia pestis inoculation.- Intranasal Inoculation of Mice with Yersinia pestis and Processing of Pulmonary Tissue for Analysis.- Standardized method for aerosol challenge of rodents with Yersinia pestis for modeling primary pneumonic plague.- Mouse Models of Yersiniosis.- Intravital Confocal Microscopy of Dermal Innate Immune Responses to Flea-transmitted Yersinia pestis.- Laser scanning microscopy of Yersinia pestis infected tissues.- Real-time monitoring of Yersinia pestis promoter activity by bioluminescence imaging.- Discovering Yersinia-Host Interactions by Tissue Dual RNA-seq.- Detection of cells translocated with Yersinia Yops in infected tissues using β-lactamase fusions.- Monitoring of neutrophil recruitment to mice lungs during pneumonic plague.- Co-infection assay to determine Yersinia pestis competitive fitness in fleas.- Drosophila as a model for understanding the insect host of Yersinia pestis.- Intracellular assays to monitor survival and growth of Yersinia pestis within macrophages.- Human neutrophil isolation and degranulation responses to Yersinia pestis infection.- Measurement of Yersinia translocon pore formation in erythrocytes.- Analysis of inflammasome activation in response to Yersinia infection by fluorescence microscopy detection of active caspase-1 puncta.- Methods for detection of pyrin inflammasome assembly in macrophages infected with Yersinia spp.