Neisseria gonorrhoeae

1. Biology of the Gonococcus: Disease and Pathogenesis.- 2. How to identify Exposed Women who are infected with Neisseria gonorhoeae.- 3. Antimicrobial resistance in Neisseria gonorrhoeae and treatment of gonorrhoea.- 4. Prediction of minimum inhibitory concentrations of antimicrobials for Neisseria gonorrhoeae using whole-genome sequencing.- 5. Gonococcal Colony Typing.- 6. Preparation of lipooligosaccharide (LOS) from Neisseria gonorrhoeae.- 7. Purification of Type IV Pili and Pilin Subunits.- 8. Peptidoglycan Composition in Neisseria.- 9. The isolation of naturally released gonococcal outer membrane vesicles as vaccine antigens.- 10. Transformation in Neisseria gonorrhoeae.- 11. Strategies for Global RNA Sequencing of the Human Pathogen Neisseria gonorrhoeae.- 12. Bioinformatics Workflow for Gonococcal Proteomics.- 13. Phenotypic MicroArray screening of Neisseria gonorrhoeae in chemically defined liquid medium.- 14. Generation of metal-depleted conditions for in vitro growth of Neisseria gonorrhoeae.- 15. Cloning, expression and purification of recombinant Neisseria gonorrhoeae proteins.- 16. Complement-dependent serum bactericidal assays for Neisseria gonorrhoeae.- 17. Basic methods for examining Neisseria gonorrhoeae interactions with host cells in vitro.- 18. Use of Human Monocyte-Derived Macrophages to Study Neisseria gonorrhoeae Infection.- 19. Protocols to interrogate the interactions between Neisseria gonorrhoeae and primary human neutrophils.- 20. Analysis of Host Responses to Neisseria gonorrhoeae using a Human Three-Dimensional Endometrial Epithelial Cell Model.- 21. In vitro models of eye infection with Neisseria gonorrhoeae.- 22. Use of Human Fallopian Tube Organ in Culture (FTOC) and Primary Fallopian Tube Epithelial Cells (FTEC) to Study the Biology of Neisseria gonorrhoeae infection.- 23. A Natural Mouse Model for Neisseria Persistent Colonization.- 24. Female Mouse Model of Neisseria gonorrhoeae Infection.- 25. Experimental Human Infection with Neisseria gonorrhoeae.