mRNA Processing

Detection and Identification of Uncapped RNA by Ligation-Mediated Reverse Transcription Polymerase Chain Reaction.- Preparation of Splicing Competent Nuclear Extract from Mammalian Cells and In Vitro Pre-mRNA Splicing Assay.- Single-Molecule Analysis of Pre-mRNA Splicing with Colocalization Single-Molecule Spectroscopy (CoSMoS).- In Vitro Modulation of Endogenous Alternative Splicing Using Splice-Switching Antisense Oligonucleotides.- Purification of mRNA Processing Complexes Using an RNA Affinity Approach.- Polyadenylation Site-Based Analysis of Transcript Expression by 3’READS+.- Comprehensive Identification of mRNA Polyadenylation Sites by PAPERCLIP.- Microinjection and Fluorescence In Situ Hybridization Assay for Studying mRNA Export in Mammalian Cells.- Methods for the Detection of Adenosine-to-Inosine Editing Events in Cellular RNA.- Using RNA-Seq to Discover Genetic Polymorphisms that Produce Hidden Splice Variants.- Inducible Expression of Eukaryotic Circular RNAs from Plasmids.- Methods for Extraction of RNA, Proteins, or Protein Complexes from Subcellular Compartments of Eukaryotic Cells.- Isolation of Newly Transcribed RNA Using the Metabolic Label 4-Thiouridine.- Robust, Cost-Effective Profiling of RNA Binding Protein Targets with Single-End Crosslinking and Immunoprecipitation (seCLIP).- Purification of Transcript-Specific mRNP Complexes Formed In Vivo from Saccharomyces cerevisiae.- A Cell-Based High-Throuput Method for Identifying Modulators of Alternative Splicing.- Genome-Wide RNAi Screens for RNA Processing Events in Drosophila Melanogaster S2 Cells.- Assaying RNA Structure Inside Living Cells with SHAPE.