Bacterial Regulatory RNA

Workflow for a Computational Analysis of an sRNA Candidate in Bacteria.- Molecular Phylogenomics for the Identification and Characterization of trans-encoded Small Non-coding RNA Families.- Bioinformatic Approach for Prediction of CsrA/RsmA-regulating small RNAs in Bacteria.- Host-pathogen Transcriptomics by Dual RNA-seq.- Identification of New Bacterial Small RNA Targets using MS2 Affinity Purification Coupled to RNA Sequencing.- Assessment of External Guide Sequences’ (EGS) Efficiency as Inducers of RNase P-mediated Cleavage of mRNA Target Molecules.- Evaluating the Effect of Small RNAs and Associated Chaperones on Rho-dependent Termination of Transcription in vitro.- A Method to Map Changes in Bacterial Surface Composition Induced by Regulatory RNAs.- Fluorescence-based Methods for Characterizing RNA interactions in vivo.- Mutational Analysis of sRNA-mRNA Base Pairing by Electrophoretic Mobility Shift Assay.- An Integrated Cell-free Assay to Study TranslationRegulation by Small Bacterial Non-coding RNAs.- Quantitative Super-resolution Imaging of Small RNAs in Bacterial Cells.- Extraction and Analysis of RNA Isolated from Pure Bacteria—Derived outer Membrane Vesicles.- Absolute Regulatory Small Noncoding RNA Concentration and Decay Rates Measurements in Escherichia coli.- High-resolution, High-throughput Analysis of Hfq Binding Sites using UV-crosslinking and Analysis of cDNA (CRAC).- Producing Hfq/Sm Proteins and sRNAs for Structural and Biophysical Studies of Ribonucleoprotein Assembly.- Single Molecule FRET Assay to Observe the Activity of Proteins Involved in RNA/RNA Annealing.- Techniques to Analyse sRNA-protein Cofactor Self-assembly in vitro.- Sequence-specific Affinity Chromatography of RNA-binding Proteins from Bacterial Cells.- Identification of Small RNA-protein Partners in Plant Symbiotic Bacteria.- A Modular Genetic System for High-throughput Profiling and Engineering of Multi-target Small RNAs.